白蜡属种间杂交子代木质素含量变异及FmPAL核苷酸多态性关联分析

Variation of Lignin Content and Association Analysis of FmPAL Nucleotide Polymorphism in Progenies of Interspecific Hybrids of Fraxinus

研究水曲柳(Fraxinus mandshurica)×大叶白蜡(Fraxinus rhynchophylla)、水曲柳×小叶白蜡(Fraxinus sogdiana)和水曲柳×绒毛白蜡(Fraxinus velutina)的3个杂交组合与母本自由授粉子代木质素含量的遗传变异及FmPAL核苷酸多态性相关性,为水曲柳遗传育种和定向改良奠定基础。以东北林业大学帽儿山林场白蜡属种间杂交组合及母本自由授粉子代共176个无性系的水曲柳当年新生枝为材料,测定木质素的含量,进行木质素含量差异、变异参数及均值比较分析,并对木质素及其合成相关基因PAL进行分子水平的联合分析。结果表明:白蜡属各杂交组合间木质素含量差异极显著,各杂交组的木质素含量均与母本自由授粉子代水曲柳差异显著。其中大多数杂交子代水曲柳的木质素含量高于母本水曲柳木质素平均含量。本研究中的水曲柳群体的PAL基因单倍体多样性值达到了0.9997,属高水平,证实了FmPAL基因具有较高的变异,且存在3个单核苷酸多态性(SNP)位点与木质素含量显著相关。主要结论:水曲柳各杂交组合的木质素含量存在显著性差异,Fm⁃PAL基因中SNP位点与木质素含量显著相关。

To improve materials for genetic breeding of Fraxinus mandshurica,the correlation among three hybrid combinations of Fraxinus mandshurica×F.rhynchophylla,F.mandshurica×F.sogdiana and F.mandshurica×F.velutina,and the genetic variation of lignin content and FmPAL nucleotide polymorphism in free-pollination offspring of female parent were analyzed respectively.One-year-old branches of Fraxinus mandshurica with 71 species of interspecies hybrid combinations in Maoer Mountain Forest Farm of Northeast Forestry University were used as materials,the genetic variation regularity of lignin was investigated,the differences in lignin content and variation parameters and mean values were analyzed,and joint analysis on the molecular level of lignin and its synthesis-related gene PAL were carried out.The result showed that the lignin content of the hybrid combinations of Fraxinus was extremely significant,and it was significantly different from the female parent free-pollinated offspring of F.mandshurica.The lignin content of most hybrid combinations of F.mandshurica was higher than that of the female parent F.mandshurica.The results showed that the haploid diversity value of the PAL gene of the F.mandshurica population reached 0.9997,which was a high level.It confirmed that the FmPAL gene had a high variation.There were 3 SNP sites significantly related to the lignin content.There were significant differences in the lignin content of the cross combinations of F.mandshurica.The SNP sites in the FmPAL gene was significantly related to the lignin content.