体外模拟子宫微环境诱导大鼠胎盘间充质干细胞分化为子宫内膜上皮细胞模型建立

Modeling of induction of placental mesenchymal stem cells of rats differentiating into endometrial epithelial cells in vitro by simulated uterine microenvironment

目的:研究模拟子宫微环境诱导大鼠胎盘间充质干细胞(PMSCs)向子宫内膜上皮细胞分化模型。方法:原代培养大鼠PMSCs及传代,绘制细胞生长曲线,以子宫内膜条件培养液及β-雌二醇体外诱导分化PMSCs,检测细胞的波形蛋白、角蛋白、CK7、CK18、CK19、EMA基因表达。结果:大鼠PMSCs增殖能力较强,使用子宫内膜诱导培养液和1×10^(-7) mol/Lβ-雌二醇培养PMSCs后,可见PMSCs细胞核呈梭形或扁平、分化为排列成团、核圆而大的子宫内膜上皮细胞,即细胞形态由间质细胞向子宫内膜上皮细胞分化,且诱导分化后子宫内膜上皮细胞表达波形蛋白和角蛋白,CK7、CK18、CK19、EMAmRNA表达提高,波形蛋白表达(0.32±0.16)降低、角蛋白表达(2.91±1.07)提高(P<0.05)。结论:子宫内膜诱导培养液与雌激素能够促进大鼠PMSCs分化为子宫内膜上皮细胞。

Objective:To study the modeling of induction of placental mesenchymal stem cells(PMSCs)of rats differentiating into endometrial epithelial cells in vitro by simulated uterine microenvironment.Methods:PMSCs of primary rats were cultured and then were gone down to the future generation.The growth curves of these cells were drawn.PMSCs were induced differentiation in vitro by endometrial conditioned culture solution andβ-estradiol.The expressions of vimentin,keratin,CK7,CK18,CK19,and EMA genes in PMSCs were detected.Results:PMSCs of rats had strong proliferation ability.After PMSCs were cultured by endometrial conditioned culture solution andβ-estradiol(1×10^(-7) mol/L),PMSCs with fusiform or flat nuclei had been differentiated into endometrial epithelial cells with clustered round and large nuclei,that was,the cell morphology differentiates from mesenchymal cells to endometrial epithelial cells.And the endometrial epithelial cells expressed vimentin and keratin after induced differentiation,and the expression of CK7,CK18,CK19,EMAmRNA,and keratin of which had increased significantly,but the vimentin expression of which had decreased significantly(P<0.05).Conclusion:The endometrial induction culture solution and estradiol can promote the PMSCs differentiating into endometrial epithelial cells.