安徽地区冠心病人群冠状动脉病变特征与载脂蛋白E基因多态性的研究

Study on the correlation between the characteristics of coronary artery and apolipoprotein E gene polymorphism in patients with coronary heart disease in Anhui province

目的研究安徽地区冠心病人群冠状动脉病变特征与载脂蛋白E(ApoE)基因多态性的相关性,评估其在冠心病诊疗及预防中的临床应用价值。方法收集2019年9月至2020年9月在合肥市第二人民医院心内科住院行冠状动脉造影检查的患者共199例,根据研究内容不同采用两种分组方式(分别是冠心病组和对照组,ε2、ε3和ε4组),采用PCR-荧光探针法检测ε2、ε3和ε4等位基因以及rs7412和rs429358的基因型。通过比较等位基因ε2、ε3、ε4携带者的冠状动脉病变特征,分析ApoE基因型/亚型与冠心病的发病及严重程度之间的关系。结果ε2、ε3和ε4在样本中的频率分别为13.6%、69.3%和17.1%,ApoE基因多态性与冠心病的发生有相关性(ε2:r=0.160,P<0.05;ε4:r=0.154,P<0.05);ε2、ε3和ε4组Gensini评分差异有统计学意义(P=0.026,0.013,0.001),犯罪血管狭窄程度和病变血管支数与ApoE基因多态性差异无统计学意义(ε2=0.15,ε3=0.822,ε4=0.276;ε2=0.324,ε3=0.849,ε4=0.233);犯罪血管病变类型中ε4携带者以局限性为主,差异有统计学意义(P=0.027)。结论 ApoE基因多态性与冠心病的发生具有良好的相关性,在安徽地区冠心病人群中,冠状动脉病变严重程度及犯罪血管病变类型与其相关。

Objective To study the correlation between the characteristics of coronary artery disease and apolipoprotein E(ApoE) gene polymorphism in patients with coronary heart disease in Anhui area.Methods A total of 199 patients who underwent coronary angiography in the Department of Cardiology,Hefei Second People’s Hospital from September 2019 to September 2020 were collected.and were divided into two groups according to the study content(coronary heart disease group and control group,respectively,ε2,ε3 and ε4 groups).PCRfluorescence probe method was used to detect the alleles of ε2,ε3 and ε4,and the genotype of RS7412 and RS429358.The relationship between ApoE genotype/subtype and the incidence and severity of coronary heart disease was analyzed by comparing the characteristics of coronary artery disease in ε2,ε3,and ε4 carriers.Results The frequencies of ApoE gene polymorphism in ε2,ε3 and ε4 were 13.6%,69.3% and 17.1%,respectively.ApoE gene polymorphism was correlated with the incidence of coronary heart disease(ε2:r=0.160,P<0.05;ε4:r=0.154).There was significant difference in gensini score in ε2,ε3 and ε4 groups(P=0.026,0.013,0.001),but there was no significant difference between the degree of criminal vascular stenosis and the number of diseased vessels and ApoE gene polymorphism(ε2=0.15,ε3=0.822,ε4=0.276;ε2=0.324,ε3=0.849,ε4=0.233).Among the types of criminal vascular lesions,ε4 carriers were mainly limited and the difference was statistically significant(P=0.027).Conclusions Apo E gene polymorphism has a good correlation with the occurrence of coronary heart disease.In the CHD population in Anhui area,it is related to the severity of coronary artery disease and the type of criminal vascular disease.