白茶多酚对D-半乳糖致小鼠肾损伤模型的改善作用

Effect of polyphenols in white tea on kidney injury induced by D-galactose in mice

该研究建立D-半乳糖诱导小鼠肾损伤模型,用不同剂量[250、500 mg/(kg·d)]白茶多酚灌胃小鼠42 d,于第6周末测量小鼠肾组织中谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)、过氧化氢酶(catalase,CAT)、总抗氧化能力(total antioxidant capacity,T-AOC)、一氧化氮(nitric oxide,NO)、丙二醛(malondialdehyde,MDA)和细胞因子IL-6、IL-10、TNF-α、IFN-β、IFN-γ的含量。组织学分析肾脏切片病理变化,体外抗氧化试验考察白茶多酚对DPPH自由基、ABTS阳离子自由基清除能力以及肾脏器官系数的分析。此外,通过HPLC分析白茶多酚的生物活性成分。结果表明,白茶多酚显著(P<0.05)提高CAT、GSH-Px、T-AOC、IL-10、IFN-β含量,降低MDA、NO、IL-6、TNF-α、IFN-γ含量,使肾脏细胞排列顺序更为规则,且对DPPH自由基以及ABTS阳离子自由基具有明显的清除效果,也能有效抵抗由D-半乳糖导致的小鼠肾脏萎缩。同时,HPLC分析发现白茶多酚里面含有隐绿原酸、没食子儿茶素没食子酸酯、表儿茶素、芦丁、山柰苷、对香豆酸6种活性物质。总之,白茶多酚具有较好的抗氧化活性,能有效改善由D-半乳糖诱导的小鼠肾损伤,其药理价值可进一步深入研究。

To investigate whether white tea polyphenols can ameliorate kidney injury in mice,the kidney damaged mice induced by D-galactose model was established,then treated with different concentrations[250,500 mg/(kg·d)]of white tea polyphenols lavage mouse model for 42 d.The contents of GSH-Px,CAT,T-AOC,NO,MDA and cytokines IL-6,IL-10,TNF-α,IFN-βand IFN-γwere measured in the kidney of mice at the end of the 6 th week.Histology(HE staining)was used to analyze the pathological changes of kidney sections.Antioxidant test in vitro was used to investigate the removal ability of polyphenols to DPPH and ABTS.The renal organ coefficient were also been analyzed.In addition,the HPLC were used to analyze bioactive components of white tea polyphenols.The results showed that polyphenols of white tea(P<0.05)significantly improved the contents of CAT,GSH-PX,T-AOC,IL-10 and IFN-βand reduced the contents of MDA,NO,IL-6,TNF-αand IFN-γ.It also made the sequence of renal cells more regular.Moreover,its apparent effect on scavenged DPPH and ABTS free radicals,and could effectively resist the renal atrophy caused by D-galactose in mice.Furthermore,HPLC analysis showed that polyphenols of white tea contained six active substances which were cryptochlorogenic acid,epigallocatechin gallate,l-epicatechin,rutin,kaempferitrin and p-coumaric acid.In conclusion,polyphenols in white tea have a good antioxidant activity and can effectively improve kidney injury induced by D-galactose in mice,and their pharmacological value could be further studied.