miR-1307-3p通过靶向ISM1促进乳腺癌细胞的增殖和迁移

MiR-1307-3p promotes the proliferation and migration of breast cancer cells by targeting ISM1

目的探讨miR-1307-3p通过靶向ISM1促进乳腺癌细胞增殖和迁移的分子机制。方法 (1)利用TCGA数据库分析miR-1307-3p在乳腺癌患者中的表达水平及其与临床指标的关系。(2)利用qPCR、CCK-8实验、细胞划痕实验和流式细胞术检测过表达或沉默miR-1307-3p后乳腺癌MCF-7细胞miR-1307-3p表达、细胞增殖、迁移和凋亡水平变化。(3)生物信息学分析软件预测miR-1307-3p的靶基因,同时采用双荧光素酶实验进行验证。结果miR-1307-3p在乳腺癌细胞及乳腺癌组织中均显著上调。miR-1307-3p过表达可促进MCF-7细胞增殖和迁移;而miR-1307-3p inhibitor则抑制细胞迁移,并诱导凋亡。ISM1可能是miR-1307-3p的靶基因。乳腺癌组织中ISM1表达水平明显低于正常乳腺组织,且与临床病理分期有关。结论 miR-1307-3p可促进乳腺癌细胞增殖和迁移,可能通过调控ISM1基因而影响乳腺癌的发生发展。

Objective To explore the possible molecular mechanism of miR-1307-3 p in promoting proliferation and migration by targeting ISM1 in breast cancer cells.Methods(1) Cancer Genome Atlas(TCGA) database was used to analyze the expression level of miR-1307-3 p and its relationship with clinical indicators in breast cancer patients.(2)qPCR,CCK-8 assay,Scratch test and flow cytometry were used to detect the effects of miR-1307-3 p over-expression or silence on the expression of miR-1307-3 p,cell proliferation,migration and apoptosis in breast cancer MCF-7 cells,respectively.(3) The target gene of mir-1307-3 p was predicted by bioinformatics analysis,and verified by double luciferase reporter gene experiment.Results The relative expression levels of miR-1307-3 p were significantly up-regulated both in breast cancer cell lines and breast cancer tissues.Overexpression of miR-1307-3 p significantly promoted cell proliferation and migration in MCF-7 cells;on the contrary,miR-1307-3 p inhibitor significantly inhibited cell migration and induced cell apoptosis.ISM1 may be the target genes of miR-1307-3 p.The expression level of ISM1 was significantly lower in breast cancer tissues than that in normal breast tissues,and was significantly correlated with clinical stages.Conclusion MiR-1307-3 p can promote the proliferation and migration of breast cancer cells,which may affect the occurrence and development of breast cancer by targeting ISM1 gene.