卵泡液中SCF和SDF-1水平与POI的发生及胚胎发育的相关性研究

Correlation study between the SCF and SDF-1 in follicular fluid and embryonic development of POI

目的研究分析原发性卵巢功能不全(POI)患者卵泡液中干细胞因子(SCF)和基质细胞衍生因子-1(SDF-1)的相关性,以及SCF与胚胎发育的关系。方法纳入接受常规体外受精(IVF)治疗的不孕患者144例并将其分为POI组(44例)、高龄组(年龄≥40岁,40例)和正常组(年龄<40岁,60例)。采用酶联免疫吸附试验(ELISA)法检测卵泡液中SCF、SDF-1和E2水平,比较分析不同组内SCF、SDF-1和E2相关性。将POI组根据卵泡液中SCF水平分为3个亚组,比较亚组间正常受精率、卵裂率、优胚率差异。结果POI组、高龄组的卵泡液SCF水平高于正常组,POI组SDF-1、E2低于正常组,差异有统计学意义(P<0.05)。POI组卵泡液中SCF与SDF-1水平呈负相关(r_(s)=-0.400,P<0.05),与E2水平呈正相关(r_(s)=0.341,P<0.05)。POI组内SCF<200 ng/L组、200~300 ng/L组的优胚率均低于>300 ng/L组,差异有统计学意义(P<0.05)。结论SCF和SDF-1可能通过相互作用共同参与了POI的发生发展,且SCF可能是预测胚胎发育的重要生化指标。

Objective To analyze the correlation between stem cell factor(SCF)and stromal cell-derived factor-1(SDF-1)in follicular fluid of patients with primary ovarian insufficiency(POI),and the correlation between SCF and embryonic development.Methods A total of 144 infertile patients who underwent routine in vitro fertilization(IVF)treatment in our center were selected.According to the diagnosis,they were divided into the POI group(n=44),the elderly group(≥40 years old,n=40)and the normal group(<40 years old,n=60).The levels of SCF,SDF-1 and E2 in follicular fluid were detected by ELISA.The correlation between SCF,SDF-1 and E2 was analyzed in different groups.The POI group was divided into three subgroups according to the SCF concentration.The differences in normal fertilization rate,cleavage rate and excellent embryo rate were compared between POI subgroups.Results The SCF level of follicular fluid was significantly higher in the POI group and the elderly group than that in the normal group(P<0.05).SDF-1 and E2 were significantly lower in the POI group than those in the normal group(P<0.05).SCF was negatively correlated with SDF-1(r_(s)=-0.400,P<0.05)and positively correlated with E2(r_(s)=0.341,P<0.05)in follicular fluid of the POI group.In the POI subgroups,the excellent embryo rates of the<200 ng/L group and the 200-300 ng/L group were significantly lower than those of the>300 ng/L group(P<0.05).Conclusion SCF and SDF-1 may interact with each other to participate in the development of POI,and SCF may be an valuable biochemical marker to predict embryonic development.