一种改良机械性牵拉血管痉挛模型的建立

Establishment of a new mechanical traction vasospasm model

目的建立一种改良的机械性牵拉血管痉挛模型,模拟重建术后的血管痉挛。方法48只Sprague-Dawley大鼠随机分为6组:血管牵拉组(拉力分别是5、10、15、20及25 g);血管钝性剥离组(对照组)。激光散斑血流成像仪监测血管平均血流灌注,收集痉挛持续时间和达到超灌注所需时间作为统计参数。结果拉力25 g组痉挛程度过强,剔除研究。拉力5 g组和10 g组血管痉挛持续时间和超灌注时间较短,与对照组比较差异有统计学意义(P<0.05);拉力15 g组与对照组比较差异无统计学意义(痉挛持续时间P=0.801;超灌注时间P=0.629);拉力20 g组与对照组比较,痉挛持续时间差异有统计学意义(P=0.014),超灌注时间差异无统计学意义(P=0.081)。结论双向15 g拉力诱发的动脉痉挛能很好地模拟临床血管剥离状态,是一种可靠的、成功率较高的血管痉挛模型。

Objective To establish a mechanical traction vasospasm model,for simulating vasospasm post-surgery.Methods Forty-eight Sprague-Dawley rats were randomly divided into 6 groups:Group 1-5 was vascular traction groups(traction force was 5,10,15,20 and 25g,respectively);Group 6 was blunt vascular dissection group(control group).Laser Speckle contrast imaging was used to monitor the average blood flow perfusion,and to collect the duration of spasm and the time needed to achieve hyperperfusion as statistical parameters.Results Group 5 had hyperspasticity and was not included in the study.The duration and hyperperfusion time of vasospasm in group 1 and group 2 were relatively short,which was statistically significant compared with the control group(P<0.05).There was no significant difference between the third group and the control group(spasm duration P=0.801;hyperperfusion time P=0.629).Compared with the control group,the fourth group showed statistically significant difference in spasm duration(P=0.014),but no statistically significant difference in hyperperfusion duration(P=0.081).Conclusions Vasospasm induced by bidirectional tension of 15g can well simulate clinical vascular dissection,and it is a reliable model with high success rate.