基于HPLC强力定眩片指纹图谱分析方法的建立

The establishment of HPLC characteristic chromatogram analysis method for Qiangli Dingxuan tablets

为建立强力定眩片的HPLC指纹图谱,使用Lamdo Stamsil C_(18)柱(柱长250 mm,柱径4.6 mm,粒径5μm);乙腈-0.05%磷酸水为流动相,梯度洗脱;波长280 nm;体积流量0.8 mL/min;进样量5μL。10批样品共标定34个共有峰,指认出12个色谱峰,相似度均在0.9以上,表明该方法准确可靠,对强力定眩片的质量评价具有重要意义。

To establish the HPLC fingerprint of Qiangli Dingxuan tablets,Lamdo Stamsil C_(18) column(250×4.6 mm,5μm)was used,using acetonitrile-0.05%phosphoric acid solution as the mobile phase of gradient elution.The detection wavelength was 280 nm,flow rate was 0.8 mL/min,and the injection volume was 5μL.There were 34 common peaks in 10 batches of samples,and 12 chromatographic peaks were identified with similarity greater than 0.9,which indicated that the method was accurate and reliable,and had important significance for quality evaluation of Qiangli Dingxuan tablets.