超声波辅助双水相提取枇杷叶中的黄酮及其抗氧化研究

Ultrasonic-assisted two-phase extraction of flavonoids from loquat leaves and their antioxidant activity

研究采用超声波辅助乙醇-硫酸铵双水相体系提取枇杷叶中的黄酮,采用羟自由基和DPPH自由基清除体系考察其抗氧化活性。结果显示,超声辅助双水相提取枇杷叶黄酮的较优工艺为:超声温度40℃、超声时间40 min、液料比30 mL/g、硫酸铵用量8 g、超声功率270 W、乙醇浓度35%。在此条件下,黄酮的提取量为10.82 mg/g。抗氧化分析表明,在枇杷叶黄酮一定浓度范围内,抗氧化能力随着质量浓度的逐渐增加而增强。研究表明,枇杷叶黄酮具有良好的抗氧化活性,具有较好的应用价值。

The study used ultrasonic-assisted ethanol-ammonium sulfate two-phase system to extract flavonoids from loquat leaves,using DPPH·free radical and hydroxyl free radical scavenging system to investigate the antioxidant activity.The results showed that the optimal technology for ultrasonic-assisted two-phase extraction of flavonoids from loquat leaves was ultrasonic temperature 40℃,ultrasonic time 40 min,liquid-to-material ratio 30 mL/g,dosage of ammonium sulfate 8 g,ultrasonic power 270 W,ethanol concentration 35%.Under the condition,the extraction amount of flavonoids was 10.82 mg/g.Antioxidant analysis showed that within a certain concentration range of loquat leaf flavonoids,its antioxidant capacity increased with the gradual increase in mass concentration.The experiment indicates that flavonoids from loquat leaf has good antioxidant activity and good application value.