高脂饮食联合内膜损伤对动脉粥样硬化的作用及对炎症因子的影响

The Effect of High-fat Diet Combined with Intimal Injury on Atherosclerosis and its Influence on Inflammatory Factors

目的分析高脂饮食联合内膜损伤对动脉粥样硬化的作用及对炎症因子水平的影响。方法将22只新西兰大白兔随机分为正常对照组、高脂组,每组11只。高脂组行髂动脉内膜剥脱术并予高脂喂养,正常对照组行假手术并予普食喂养。喂养4、12周后行血管超声检查并检测各组血脂及炎症水平变化,比较两组内膜-中膜厚度(IMT)比值变化。结果喂养4周后,两组IMT、管腔内径、血流速度比较,差异有统计学意义(P<0.05);高脂组喂养4、12周总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)、白介素-6(IL-6)、C反应蛋白(CRP)水平高于正常对照组,差异有统计学意义(P<0.05);高脂组IMT比值为(1.865±0.557),大于正常对照组的(0.206±0.085),差异有统计学意义(P<0.05)。结论高脂饮食联合内膜损伤成功建立动脉粥样硬化模型,其炎性因子IL-6、CRP水平在血液中表达增高,参与动脉粥样硬化的发生与发展。

Objective To analyze the effect of high-fat diet combined with intimal injury on atherosclerosis and its influence on inflammatory factor levels.Methods Twenty-two New Zealand white rabbits were randomly divided into normal control group and high-fat group,with 11 rabbits in each group.In the high-fat group,the iliac artery intima was stripped and fed with high-fat diet.In the normal control group,the sham operation was performed and fed with normal diet.After 4 and 12 weeks of feeding,vascular ultrasound was performed to detect the changes of blood lipids and inflammation levels in each group,and the changes of intima-media thickness(IMT)ratio between the two groups were compared.Results There were significant differences in IMT,lumen diameter and blood flow velocity between the two groups(P<0.05).The levels of total cholesterol(TC),triglyceride(TG),low-density lipoprotein cholesterol(LDL-C),interleukin-6(IL-6)and C-reactive protein(CRP)in the high-fat group at 4 and 12 weeks after feeding were higher than those in the normal control group,and the difference was statistically significant(P<0.05).The IMT ratio of high fat group was(1.865±0.557),which was higher than that(0.206±0.085)of normal control group,and the difference was statistically significant(P<0.05).Conclusion High-fat diet combined with intimal injury successfully established an atherosclerosis model.Inflammatory factors IL-6 and CRP are increased in the blood and involved in the occurrence and development of atherosclerosis.