Xpert MTB/RIF在肺结核病及耐药结核菌检测中的应用价值

Application Value of Xpert MTB RIF in the Detection of Pulmonary Tuberculosis and Drug-resistant Tuberculosis

目的研究Xpert MTB/RIF在肺结核病及耐药结核菌检测中的应用价值。方法选取2018年1月-2020年12月在我院诊治的100例肺结核病患者临床资料,均采用固体培养、抗酸染色法、Xpert MTB/RIF检测方法检测患者痰液标本,比较不同检测方法检测痰液标本中结核杆菌及耐药菌阳性率以及抗酸染色法和Xpert MTB/RIF检测方法敏感度、特异度。结果Xpert MTB/RIF阳性率(72.00%)高于固体培养法(56.00%)、抗酸染色法(34.00%),且固体培养法的阳性率高于抗酸染色法,差异有统计学意义(P<0.05);Xpert MTB/RIF检测结核杆菌敏感度为82.50%、特异度为88.34%,分别高于抗酸染色法的68.50%、53.02%,差异有统计学意义(P<0.05)。结论肺结核病及耐药结核菌检测采用Xpert MTB/RIF检测方法,不仅操作简单,而且敏感度、特异度均较高,值得临床应用。

Objective To study the value of XPERT MTB/RIF in the detection of tuberculosis and drug-resistant tuberculosis.Methods The clinical data of 100 patients with pulmonary tuberculosis diagnosed and treated in our hospital from January 2018 to December 2020 were selected.The sputum samples of patients were detected by solid culture,acid-fast staining and Xpert MTB/RIF detection methods.The positive rates of tuberculosis and drug-resistant bacteria in sputum samples detected by different detection methods were compared,and the sensitivity and specificity of acid-fast staining and Xpert MTB/RIF detection methods were compared.Results The positive rate of Xpert MTB/RIF(72.00%)was higher than that of solid culture method(56.00%)and acid-fast staining method(34.00%),and the positive rate of solid culture method was higher than that of acid-fast staining method,the difference was statistically significant(P<0.05).The sensitivity and specificity of Xpert MTB/RIF for detection of Mycobacterium tuberculosis were 82.50%and 88.34%,respectively,which were higher than 68.50%and 53.02%of acid-fast staining,and the difference was statistically significant(P<0.05).Conclusion The XPERT MTB/RIF method for detection of clinical tuberculosis and drug-resistant tuberculosis is not only simple in operation,but also highly sensitive and specific,which has significant clinical value and is worthy of clinical application.