厌氧膨胀颗粒污泥床生物膜反应器同步产甲烷-硫酸盐还原的快速启动

Rapid start-up of simultaneous methanogenesis and sulfate reduction in anaerobic expanded granular sludge blanket biofilm reactor

采用新型厌氧膨胀颗粒污泥床生物膜反应器(EGSBBR)处理含硫酸盐有机废水。在不添加硫酸盐的条件下,运行30 d后系统产甲烷成功启动;随后逐步提高硫酸盐质量浓度至100 mg/L,运行71 d后系统COD去除率和SO;去除率分别达98.1%和74.7%,甲烷产生量为470.7 mL/d,成功实现了同步产甲烷-硫酸盐还原耦合过程。反应器中污泥和生物膜的胞外聚合物(EPS)特性与甲烷生成量、SO;去除率具有直接关联。高通量测序发现体系内甲烷丝菌(Methanothrix sp.)等产甲烷菌和脱硫弧菌(Desulfovibrio sp.)、硫磺单胞菌(Sulfurospirillum sp.)等硫酸盐还原菌优势共存。

A novel anaerobic expanded granular sludge blanket biofilm reactor (EGSBBR) was used to treat organic sulfate-containing wastewater.Without sulfate addition,the methanogenesis of the system was successfully started up after 30-d operation;Subsequently,as the sulfate mass concentration was gradually increased to 100 mg/L in 71-d operation,the highest removal rate of COD and SO;reached 98.1% and 74.7%,and the yield of methane was 470.7 mL/d,which indicated that the combination process of simultaneous methanogenesis and sulfate reduction was realized.The characteristics of extracellular polymeric substance (EPS) in the sludge and biofilm in the reactor were directly correlated to the methane yield and SO;removal rate.The High-throughput sequencing results show that methanogens such as Methanothrix sp.coexist with sulfate reducting bacteria such as Desulfovibrio sp.and Sulfurospirillum sp.in the system.