脉冲电场对好氧反硝化细菌生长代谢的影响

Effect of Pulsed Electric Field on Growth Metabolism of Aerobic Denitrifiers

将脉冲电场技术应用于硝酸盐废水的好氧反硝化处理.通过比较不同脉冲电场强度、脉冲频率、极板间距和接种量等因素作用下好氧反硝化细菌生长代谢的变化情况,确定了脉冲电场处理的最佳工艺参数.结果表明,在电场强度为0.8 V·cm^(-1),频率为1000 Hz,极板间距为5 cm,接种量为5%的最佳试验条件下,经脉冲电场处理的好氧反硝化细菌Pseudomonas putida W207-14仅24 h其在600 nm波长下的细胞光密度(OD600)、化学需氧量(COD)和硝态氮(NO_(3)^(-)-N)的去除率均达到最大值,分别为1.926±0.04,(97.67±1.12)%和(90.34±0.73)%.与未处理的好氧反硝化细菌相比,在生长时间大幅缩短的同时,其硝酸盐去除速率提高了115.76%.

Pulsed electric field(PEF)technology was applied to aerobic denitrification treatment of nitrate wastewater.By comparing the changes of growth metabolism of aerobic denitrifiers under different PEF strength,frequency,electrode distance and inoculation volume,the optimal process parameters of PEF treatment were determined.The results indicated that under the conditions of PEF strength 0.8 V·cm^(-1),frequency 1000 Hz,electrode distance 5 cm and inoculation volume 5%,the optical density at 600 nm wavelengths(OD600),removal efficiency of COD and NO_(3)^(-)-N of aerobic denitrifiers Pseudomonas putida W207-14 treated with PEF can reach the maximum value in 24 h,which were 1.926±0.04,(97.67±1.12)%and(90.34±0.73)%,respectively.Compared with the untreated aerobic denitrifiers,the growth time of the treated aerobic denitrifiers is significantly reduced,and the nitrate removal rate is increased by 115.76%.