Apelin-13、PTX-3、GDF-15在急性肺栓塞患者中的水平及与预后的相关性研究

Levels of Apelin-13,PTX-3 and GDF-15 in patients with acute pulmonary embolism and their correlation with prognosis

目的探讨内源性配体-13(Apelin-13)、正五聚蛋白-3(PTX-3)、生长分化因子-15(GDF-15)在急性肺栓塞(APE)患者中的水平及与患者预后的相关性。方法选取2019年2月至2021年2月江苏省丹阳市人民医院收治的188例APE患者作为观察组,根据临床结局分为存活组、死亡组2个亚组。另外选取同期72例体检健康者作为对照组。采用酶联免疫吸附试验(ELISA)检测Apelin-13、PTX-3、GDF-15水平,分析Apelin-13、PTX-3、GDF-15水平与APE患者预后的相关性及其对APE的诊断价值。结果与对照组比较,观察组Apelin-13、PTX-3、GDF-15水平升高(P<0.05)。Apelin-13、PTX-3、GDF-15水平随着疾病严重程度的增加而升高,差异有统计学意义(P<0.05)。与死亡组比较,存活组Apelin-13、PTX-3、GDF-15水平均较低(P<0.05)。Apelin-13、PTX-3之间呈正相关(r=0.230,P=0.026),Apelin-13、GDF-15之间呈正相关(r=0.312,P=0.002),PTX-3、GDF-15之间呈正相关(r=0.291,P=0.004)。与Apelin-13、PTX-3、GDF-15单项检测比较,三者联合检测对APE的诊断价值更高(P<0.05)。结论Apelin-13、PTX-3、GDF-15在APE患者血清中呈异常高表达且参与疾病发生、发展,三者联合检测对APE具有较高的诊断价值。

Objective To investigate the levels of endogenous ligand-13(Apelin-13),pentraxin-3(PTX-3),growth differentiation factor-15(GDF-15)in the patients with acute pulmonary embolism(APE)and their correlation with the prognosis of the patients.Methods One hundred and eighty-eight patients with APE in Danyang Municipal People′s Hospital from February 2019 to February 2021 were selected as the observation group and divided into the survival subgroup and death subgroup according to the clinical outcomes.Contemporaneous 72 healthy subjects undergoing the physical examination were selected as the control group.The levels of Apelin-13,PTX-3 and GDF-15 were detected by enzyme-linked immunosorbent assay(ELISA),and the correlation between the levels of Apelin-13,PTX-3 and GDF-15 and the prognosis of the patients with APE,and their diagnostic value for APE was analyzed.Results Compared with the control group,the levels of Apelin-13,PTX-3 and GDF-15 in the observation group were increased(P<0.05).The levels of Apelin-13,PTX-3 and GDF-15 were elevated with the increase of disease severity,and the differences were statistically significant(P<0.05).Compared with the death group,the levels of Apelin-13,PTX-3 and GDF-15 in the survival group were lower(P<0.05).The positive correlation existed between Apelin-13 and PTX-3(r=0.230,P=0.026);the positive correlation existed between Apelin-13 and GDF-15(r=0.312,P=0.002);the positive correlation existed between PTX-3 and GDF-15(r=0.291,P=0.004).Compared with Apelin-13,PTX-3 and GDF-15 single detection,the 3-item combined detection had the higher diagnostic value for APE(P<0.05).Conclusion Apelin-13,PTX-3 and GDF-15 are highly expressed in the serum of the patients with APE,which participate in the occurrence and development of the disease,and their combined detection has higher diagnostic value for APE.