磺化酞菁与人血清白蛋白复合物的光动力活性研究

Study on the photodynamic activity of sulfonated-phthalocyanine and human serum albumin complex

通过光谱法研究了三种磺化酞菁(α位四磺化酞菁、β位四磺化酞菁和α位单取代磺化酞菁)与人血清白蛋白(HSA)的相互作用。结果表明,HSA对α位四磺化酞菁的存在状态(单体、聚集体)影响显著,而对β位四磺化酞菁和α位单取代磺化酞菁的存在状态没有明显影响。磺化酞菁与HSA均存在明显的相互作用,且四磺化酞菁与HSA的结合作用较α位单取代磺化酞菁强。通过温育交换法,制备出α位四磺化酞菁ZnPcE4和HSA的非共价复合物,复合物中两者的摩尔比约为1∶1。吸收光谱表明,复合物的Q带最大吸收波长从692 nm红移至695 nm,展现出比相应的游离磺化酞菁更明显的单体特征吸收峰。光动力抗癌活性测试表明,复合物ZnPcE4-HSA的光动力抗癌活性强于相应的游离磺化酞菁,该类复合物有望发展为靶向型的抗癌光敏剂。

The interaction between three sulfonated phthalocyanines(α-tetrasulfonated phthalocyanine,β-tetrasulfonated phthalocyanine andα-monosubstituted sulfonated phthalocyanine)and human serum albumin(HSA)was studied by spectroscopic methods.The results showed that HSA had a significant effect on the existing state ofα-substituted sulfonated phthalocyanine(monomer and aggregate)and not on the existing state ofβ-substituted sulfonated phthalocyanine andα-monosubstituted sulfonated phthalocyanine.There was an obvious interaction between sulfonated phthalocyanine and HSA,and the combination of tetrasulfonated phthalocyanine and HSA was stronger thanα-monosubstituted sulfonated phthalocyanine.The noncovalent complex ofα-tetrasulfonated phthalocyanine(ZnPcE4)and HSA was prepared by incubation exchange method,and the molar ratio of ZnPcE4 and HSA in the complex was about 1∶1.The spectral study showed that the maximum absorption wavelength of Q-band shifted from 692 nm to 695 nm,showing a more obvious spectral characteristics of monomer absorption peak than the corresponding free sulfonated phthalocyanine.The photodynamic anticancer activity test showed that the photodynamic anticancer activity of the complex ZnPcE4-HSA was stronger than the corresponding free sulfonated phthalocyanine,which proved that this type of complex was expected to develop into a targeted anticancer photosensitizer.