有氧运动通过脂联素/p38MAPK延缓高脂膳食大鼠肝脏炎症

Aerobic exercise retarded liver inflammation by adiponectin/p38MAPK in rats with high fat diet

[目的]观察有氧运动对高脂膳食大鼠肝脏炎症水平,血清脂联素和肝组织AdipoR2,p38MAPK及TNF-α蛋白表达的影响,探讨有氧运动延缓高脂膳食大鼠肝脏炎症的作用机制.[方法]SD雄性大鼠30只随机分为正常组(N组)、模型组(C组)和运动组(E组),N组喂食基础饲料,C组和E组均喂食高脂饲料,E组大鼠进行12周游泳训练.12周末处死大鼠,ELISA法检测血清脂联素、血脂和肝功能;HE染色观察各组大鼠肝组织病理变化;Western印迹法检测各组大鼠肝组织AdipoR2,p38MAPK及TNF-α蛋白表达水平.[结果]与N组相比,C组大鼠肝细胞可见脂肪弥漫性变性,炎症细胞浸润肝小叶和汇管区,肝板排列紊乱;血清TC,TG,FFA,ALT,AST水平及肝组织p38MAPK,TNF-α蛋白表达均显著升高,血清脂联素及肝组织AdipoR2蛋白水平显著降低.与C组相比,E组大鼠肝细胞形态基本正常,肝小叶及汇管区内炎症细胞浸润较少,肝板排列较整齐;血清TC,TG,FFA,ALT,AST水平及肝组织p38MAPK,TNF-α蛋白表达均显著降低,血清脂联素及肝组织AdipoR2蛋白水平显著升高.[结论]有氧运动可通过提高高脂膳食大鼠血清脂联素及肝组织AdipoR2蛋白水平,抑制肝组织p38MAPK,TNF-α蛋白的过度表达,延缓肝脏炎症的发生、发展.

[Objective]To observe the effects of aerobic exercise on the liver inflammation level,serum adiponectin,liver tissue AdipoR2,p38MAPK and TNF-αprotein expression in high-fat diet rats,and to explore the mechanism of aerobic exercise on delaying liver inflammation in high-fat diet rats.[Method]Thirty SD male rats were randomly divided into normal group(group N),model group(group C)and exercise group(group E).Group N was fed on basic diet,and group C and E were fed on high-fat diet.The rats in group E received swimming training for 12 weeks.Rats were sacrificed at the end of the 12th week,and serum adiponectin,blood lipids and liver function were detected by ELISA,the pathological changes in liver tissues of rats in each group were observed by HE staining,AdipoR2,p38MAPK and TNF-αprotein expression in liver tissues of rats in each group were detected by Western blotting level.[Result]Compared with group N,rats in group C showed that diffuse fatty degeneration of liver cells,inflammatory cells infiltrated liver lobules and portal area,liver plate arrangement disorder,serum TC,TG,FFA,ALT,AST levels and the expression of p38MAPK and TNF-αprotein in liver tissue were significantly increased,and the levels of serum adiponectin and AdipoR2 protein in liver tissue were significantly decreased.Compared with group C,rats in group E showed that the morphology of hepatocytes was basically normal,with less inflammatory cell infiltration in liver lobules and portal areas,and liver plates were arranged more neatly,serum TC,TG,FFA,ALT,AST levels and the expression of p38MAPK and TNF-αprotein in liver tissue were significantly reduced,and the levels of serum adiponectin and AdipoR2 protein in liver tissue were significantly increased.[Conclusion]Aerobic exercise can increase the levels of serum adiponectin and AdipoR2 protein in liver tissue,inhibit the over-expression of p38MAPK and TNF-αprotein in liver tissue,and delay the occurrence and development of liver inflammation of rats with high-fat diet.