MMP9、SOD、NSE联合检测对脑震荡的辅助诊断价值

Auxiliary diagnostic value of combined detection of MMP9,SOD and NSE levels in cerebral concussion

目的探讨基质金属蛋白酶-9(MMP9)、超氧化物歧化酶(SOD)、神经元特异性烯醇化酶(NSE)联合检测对脑震荡的辅助诊断价值。方法选取2018年4月至2020年8月重庆市九龙坡区人民医院收治的84例脑震荡患者为研究组,另选取同期体检的72例健康者作为对照组。采用酶联免疫吸附试验检测各组MMP9及NSE水平,采用全自动电化学发光分析仪检测SOD水平,并应用受试者工作特征(ROC)曲线分析MMP9、SOD及NSE水平对脑震荡的诊断价值。结果与对照组比较,研究组MMP9及NSE水平明显升高,而SOD水平明显降低,差异均有统计学意义(P<0.05)。经ROC曲线分析,MMP9、SOD及NSE联合检测的曲线下面积、灵敏度及特异度均高于MMP9、SOD及NSE单独检测(P<0.05)。结论与单项检测相比,MMP9、SOD及NSE 3项指标联合检测能显著提高诊断脑震荡的灵敏度及特异度,可作为临床诊断脑震荡的辅助指标。

Objective To explore the diagnostic value of combined detection of matrix metalloproteinase-9(MMP9),superoxide dismutase(SOD)and neuron-specific enolase(NSE)levels in cerebral concussion.Methods A total of 84 patients with cerebral concussion admitted to the Jiulongpo District People′s Hospital from April 2018 to August 2020 were selected as the study group,and 72 healthy people undergoing the physical examination during the same period were selected as the control group.The enzyme-linked immunosorbent assay was used to detect the levels of MMP9 and NSE in each group,and the SOD level was detected by the automatic electrochemiluminescence analyzer.The receiver operating characteristic(ROC)curve was used to analyze the diagnostic value of MMP9,SOD and NSE levels in cerebral concussion.Results Compared with the control group,the levels of MMP9 and NSE in the study group were significantly increased,while the SOD level was significantly decreased,and the differences were statistically significant(P<0.05).The receiver operating characteristic(ROC)curve analysis showed that the area under the ROC curve,sensitivity and specificity of the combined detection of MMP9,SOD and NSE were higher than those of the MMP9,SOD and NSE single detection(P<0.05).Conclusion Compared with single item detection,the combined detection of MMP9,SOD and NSE can significantly improve the sensitivity and specificity in diagnosing cerebral concussion,and can be used as the clinical auxiliary diagnosis index in cerebral concussion.