CD68因子在急性髓系白血病患者中的表达情况及其临床相关性研究

The Expression and Clinical Correlation of the CD68 Factor in Acute Myeloid Leukemia Patients

目的:分析急性髓系白血病(AML)患者骨髓及外周血血浆中CD68因子的表达及临床特点。方法:采用四色流式细胞术检测50例初诊AML患者、23例对照组(非血液系统恶性疾病)的骨髓原始细胞CD68的表达情况。采用ELISA法检测85例初诊期、29例缓解期AML患者与24例对照组外周血血浆中CD68的表达情况。分析CD68因子的表达及其与临床特征的相关性。结果:非M3型AML患者骨髓白血病细胞中CD68表达率中位数为19.7%,明显高于对照组的0.2%(P<0.001)。非M3型AML患者外周血中CD68浓度中位数为67.97 pg/ml,明显高于对照组的29.94 pg/ml(P<0.01)。配对分析非M3型AML患者初诊时(45.72 pg/ml)与缓解期(55.12 pg/ml)外周血血浆中CD68浓度差异无明显统计学意义(P>0.05)。分别比较非M3型AML骨髓中CD68的表达率、外周血中CD68浓度与白细胞计数等临床资料相关性的结果表明:骨髓中CD68表达率越高,外周血白细胞数越高,血红蛋白及血小板数越低;外周血血浆中CD68浓度越高,外周血白细胞计数越高,完全缓解率越低。结论:CD68因子在非M3型AML患者骨髓及外周血中的表达均较对照组升高。CD68高表达患者完全缓解率低,CD68表达水平与治疗反应具有相关性。

Objective:To analyze the expression and clinical characteristics of CD68 in bone marrow and peripheral blood of patients with acute myeloid leukemia(AML).Methods:The expression of CD68 in bone marrow blast cells was detected by four-color flow cytometry in 50 newly diagnosed AML patients and 23 controls.The expression of CD68 in peripheral blood of 85 newly diagnosed AML patients,29 remission AML patients and 24 controls was detected by ELISA.The correlation between the expression rate of non-M3 AML bone marrow CD68,peripheral blood CD68 concentration and white blood cell count and other clinical data was compared respectively.Results:The median CD68 expression rate in myeloid leukemia cells of non-M3 AML patients was 19.7%,significantly higher than control(0.2%)(P <0.001).The median concentration of non-M3 CD68 in peripheral blood was 67.97 pg/ml,significantly higher than in control(29.94 pg/ml)(P <0.01).There was no statistically significant difference in the plasma CD68 concentration of the peripheral blood between the newly diagnosed(45.72 pg/ml) and the remission stage(55.12 pg/ml) of non-13 AML patients by paired analysis(P>0.05).The results showed that the higher the expression rate of CD68 in bone marrow,the higher the count of white blood cells in peripheral blood,and the lower the count of hemoglobin and platelet in peripheral blood.The higher the plasma concentration of CD68 in peripheral blood,the higher the white blood cell count and the lower the complete remission rate.Conclusion:The expression of CD68 both in bone marrow and peripheral blood of patients with non-M3 AML is higher than that of control group.Patients with high expression of CD68 show a low rate of complete remission,suggesting that the expression level of CD68 is correlated with treatment response.