1867×陇薯7号优良株系的SSR及染色体构型和育性分析

Analysis of SSR,Chromosome Configuration and Fertility of 1867×Longshu 7 Hybrid Lines

为深入研究马铃薯1867×陇薯7号杂交组合的杂种F1无性株系在细胞和分子水平的差异,本试验以1867和陇薯7号为对照材料,利用SSR分析34个株系的DNA位点差异,明确选出16个优良株系的花粉育性及其染色体配对构型,为马铃薯新品种选育提供优质材料。研究表明:(1)利用筛选的2对SSR特异引物S118和STI047,能够区别出34个杂种株系和其双亲,并建立其SSR指纹图谱;(2)杂种株系C2、C3、C4、C5、C9、C10、C11、C12、C14、C19、C20、C21、C23、C26、C29和C30的花粉可育率之间存在明显差异,介于19.01%~90.59%之间,且株系C2、C3和C26显著高于母本1867;(3)16个杂种株系的染色体构型差异明显,介于1.71%~14.80%之间,其中株系C2、C3、C5、C9、C11、C12、C14、C20、C21、C26和C29二价体构型的频率较高,且以株系C26的二价体比例最高(14.80%)。本试验构建34个马铃薯株系的SSR指纹图谱,明确16个优异株系的染色体构型及花粉育性差异,为马铃薯杂交育种提供丰富的种质资源。

To deep research the differences among hybrid lines from 1867×Longshu 7 in DNA level and cytogeneti-cal level,and the test used 1867 and Longshu 7 as contrast,and analyzed DNA site difference of 34 lines by usingSSR,and the pollen fertility and chromosome configuration of 16 excellent lines selected,which could provide forbreeding qualitative materials of new potato varieties.The result showed that(1)Two SSR primers S118 and STI047 could identify 34 hybrid lines and their parents,which constructed SSR fingerprint.(2)The pollen fertility rate of hy-brid C2,C3,C4,C5,C9,C10,C11,C12,C14,C19,C20,C21,C23,C26,C29 and C30 was significantly different,ranging from 19.01%to 90.59%.In addition,line C2,C3 and C26 were significantly higher than the female parent"1867".(3)Chromosome configurations of the 16 hybrid lines was significantly different,ranging from 1.71%to14.80%.The frequencies of C2,C3,C5,C9,C11,C12,C14,C20,C21,C26 and C29 divalent configurations werehigher,and the proportion of C26 was the highest(14.80%).In this study,SSR fingerprints of 34 potato lines wereconstructed,and the differences of chromosome configuration and pollen fertility of 16 excellent lines were deter-mined,providing abundant germplasm resources for potato cross breeding.