羊草CDPK1基因的克隆及序列分析

Cloning and Sequence Analysis of CDPK1 Gene from Leymus chinensis

羊草是一种优质的天然牧草,对于盐碱胁迫有着很强的耐受性。本研究通过羊草(Leymus chinensis)EST(Expressed Sequence Tags,ESTs)序列和RACE(Rapid-amplification of cDNA Ends,RACE)技术,获得了LcCDPK1基因序列,通过生物信息学分析,得到LcCDPK1的全长基因,含1599 bp的开放读码框(Open Reading Frame,ORF),编码含有532个氨基酸的不稳定弱酸性亲水蛋白质,同时对得到的LcCDPK1基因进行了进化分析。本研究有助于了解LcCDPK基因家族的功能,为进一步应用LcCDPK基因进行植物的耐盐碱性遗传改良奠定了基础。

Leymus chinensisis an important native forage grass with strong adaptability to saline-alkali.In this study,the full-length gene of LcCDPK1 was cloned by EST and RACE,with an ORF of 1599 bp,which encodes an unstable,weakly acidic hydrophilic protein containing 532 amino acids.And a phylogenetic tree is constructed with the LcCDPK1 sequence.This study would help to further understand the function of the CDPK protein family of Leymus chinensis and provide a basis for genetic improvement of plants’saline-alkali tolerance with LcCDPK1.