氟化钠壳聚糖凝胶对乳牙釉质抗酸能力的影响

Study on enamel in primary teeth treated with NaF-chitosan gel for demineralization

目的本实验通过研究氟化钠壳聚糖温敏凝胶对乳牙牙釉质表面抗酸蚀的作用,初步探讨氟化钠壳聚糖温敏凝胶在防治早期龋中的应用可能性。方法配制氟化钠壳聚糖温敏凝胶,观察其基本性能,使用氟离子选择电极检测壳聚糖氟化钠凝胶氟离子的释放速率。制备乳前牙试件,随机分成4组,分别为多乐氟组、氟化钠壳聚糖凝胶组、壳聚糖空白凝胶处理组和空白对照组,进行乳酸脱钙实验,离子火焰法测试洗脱出的钙离子浓度。SPSS19.0对不同组钙离子浓度进行方差分析,统计结果。结果壳聚糖氟化钠凝胶,常温下为淡黄色清亮黏稠液体,37℃时转变为乳白色的果冻状凝胶。壳聚糖氟化钠凝胶4 h氟离子的释放率为70.22%。氟化钠壳聚糖凝胶处理的乳前牙釉质试件,其脱出的钙离子浓度为4.29 mg/L,低于壳聚糖空白凝胶组(7.20 mg/L)及空白对照组(7.34 mg/L),有统计学差异;但和多乐氟组(4.22 mg/L)没有统计学差异(P>0.05)。结论氟化钠壳聚糖凝胶可以提高乳前牙的釉质抗酸能力,有应用于预防乳前牙早期龋的可能性。

Objective To estimate the demineralization of anterior primary teeth pretreated by NaF-chitosan gel and to discuss the potentiality of NaF-chitosan gel for early stage caries treatment.Method NaF-chitosan gel was prepared,and its basic performance observed;NaF-chitosan gel’s F ion release rate was tested.Anterior primary teeth samples were prepared and divided into 4 groups randomly.Three groups were pretreated with Duraphat,NaF-chitosan gel and chitosan gel respectively.The 4th group was the blank control group.All samples were demineralized with lactic acid.The liquid was collected and its Ca^(2+)ion concentration was tested.The calcium ion concentration was measured by ion flame method.The variance of Ca^(2+)ion concentration in different groups was analyzed with SPSS 19.0.Results NaF-chitosan gel was light yellow clear liquid at room temperature.It changed into ivory jelly gel at 37℃.NaF-chitosan gel could release fluorine ion 70.22%in 4 h.There was no difference between NaF-chitosan gel(4.29 mg/L)and gold standard duraphat(4.22 mg/L)in reducing demineralization by lactic acid.The Ca^(2+)ion concentration was much lower in NaF-chitosan gel group than in chitosan gel(7.20 mg/L)and control group(7.34 mg/L)(P<0.05).Conclusion NaF-chitosan gel can improve the anti-acid ability of enamel in anterior teeth,and it can be used to prevent early caries of anterior teeth.