SPF级SD与Wistar大鼠不同饲养周期和性别部分生物学指标的比较分析

Comparison of some Indicators among SD and Wistar Rats in Different Feeding Periods and Genders

目的考察不同饲养周期和不同性别的SPF级SD大鼠与Wistar大鼠的部分生理及生化指标的生物学差异,建立不同品系及不同饲养周期大鼠血液生理及生化指标的正常参考值范围。方法 5~6周龄SPF级SD大鼠与Wistar大鼠各300只,雌雄各半,饲养于屏障环境中,随机分为饲养4周组、12周组及24周组,并饲养相应的周数。用全自动血细胞和生化分析仪进行血液生理及生化指标检测。结果 SD大鼠在12周组雌鼠和24周组雌、雄鼠的白细胞计数(WBC)总数显著高于4周组大鼠(P<0.01),12周组雄鼠的WBC总数高于4周组大鼠(P<0.05),4周组和12周组的雌鼠WBC总数低于雄鼠(P<0.05)。不同饲养周期和性别的Wistar大鼠血液学指标无显著差异(P>0.05)。SD大鼠4周组血清碱性磷酸酶(ALP)显著高于12周组及24周组(P<0.01),且4周组雌鼠ALP低于雄鼠(P<0.05),12周组和24周组雌鼠ALP显著低于雄鼠(P<0.01);12周组和24周组SD大鼠的甘油三酯(TG)均高于4周组(P<0.05)。Wistar雌性大鼠12周组和24周组雌、雄鼠的ALP均显著高于4周组(P<0.01),12周组雄鼠ALP高于4周组(P<0.05)。4周组和24周组Wistar雌鼠ALP显著高于雄鼠(P<0.01),12周组雌鼠ALP高于雄鼠(P<0.05)。结论不同饲养周期、不同性别及不同品系的SPF级大鼠,血液生理及生化指标具有一定差异性。

Objective In order to investigate the biological differences of some physiological and biochemical indexes between SD rats and Wistar rats in SPF level at different feeding periods and different genders,and to establish the normal reference ranges of blood physiological and biochemical indexes of different strains and ages.Method Three hundred SPF Wistar rats and 300 SD rats at the age of 5 to 6 weeks old were randomly assigned into 4,12 and 24 weeks groups by weight,with half male and half female. The blood physiological and biochemical indicators were detected by fully automatic biochemical analyzer and blood cell analyzer. Result The total WBC of female rats at 12-week group and male rats at 24-week group was significantly higher than 4-week group rats( P<0. 01). The total WBC of male rats at 12-week group was higher than 4-week group rats( P<0. 05),while the total WBC in 4-week and 12-week groups female rats was lower than that in male rats( P <0. 05). There was no significant difference in hematologic indexes of Wistar rats of different feeding period and genders( P>0. 05). The serum ALP of SD rats at 4-week group was significantly higher than that of 12-week and 24-week groups( P<0. 01),and the ALP of female rats at 4-week group was significantly lower than that of male rats( P<0. 05),and the ALP in female rats at 12-week and 24-week group was significantly lower than that in male rats( P<0. 01).TG in SD rats was higher than 4-week group( P< 0. 01). The ALP in 12-week and 24-week groups female Wistar rats was significantly higher than 4-week group rats( P< 0. 01),and 12 weeks male rats was significantly higher than 4 weeks rats( P < 0. 05). ALP of female Wistar rats at 4-week and 24-week groups was significantly higher than that of male rats( P <0. 01),and ALP of female rats at 12-week group was significantly higher than that of male rats( P< 0. 05). Conclusion Different feeding periods,genders and strains of SPF rats have some distinctions in blood physiological and biochemical indexes.