基因工程小鼠剖宫产生物净化技术的研究

Study on Purification Technology of Genetically Engineered Mice Cesarean Section

目的改进剖宫产手术,提高剖宫产生物净化效率,获得SPF级基因工程小鼠。方法结合小鼠妊娠时间,触诊判断胎鼠发育情况推测剖宫产手术时间,优化剖宫产手术流程,缩短手术时间以提高手术效率;改进剖出仔鼠由SPF级ICR小鼠代乳以提高成活率;采用统计学方法对剖宫产净化数据进行分析。结果以触摸胎鼠四肢清晰,头部与躯干长度相似,且胎位朝下为剖宫产手术最佳时间,改进手术细节使成功率达(97.9±8.25)%;选择分娩时间和手术时间间隔最小的ICR母鼠代乳,间隔时间<24 h,可提高仔鼠的离乳率,平均窝离乳率为91.2%;经剖宫产净化技术成功获得的仔鼠检测微生物级别符合SPF级。结论剖宫产净化技术的优化可显著提高实验小鼠的净化效率,为基因工程小鼠的生物净化奠定基础。

Objective To obtain SPF healthy mice and improve the purification efficiency of cesarean section.Method The following experiments are involved in this study,such as judging the development of the fetus by palpation to accurately determine the start time of the cesarean section,by optimizing the cesarean section procedure,shortening the operation time to improve the efficiency of cesarean section,improving the milking method of the excised pup rate and doing statistical method to analyze the cesarean section purification data. Result A. By optimizing and improving the surgical procedure, the success rate of uterine section surgery reached( 97. 9± 8. 25) %;B. when the time interval was less than 24 hours,the average lactation rate was 91. 2%;C. the microbiological grades of the pups purified by cesarean section technology reached the SPF level. Conclusion The perfect cesarean section technique not only improves the microbiological grade of laboratory mice,but also greatly improves the success rate of surgery,and laying the foundation for the biological purification of laboratory mice.