红花多糖对肠道微生态失调小鼠肠黏膜sIgA、血浆内毒素和肠道菌群的影响

The effect of Carthamus tinctorius polysaccharides on intestinal mucosa sIgA,plasma endotoxin and intestinal flora in mice with intestinal microecological disorders

目的研究红花多糖对肠道微生态失调小鼠的调节作用,探讨红花多糖改善微生态失调与肠黏膜免疫、血浆内毒素及肠道菌群的关系。方法应用盐酸林可霉素灌胃建立肠道微生态失调小鼠模型,然后用红花多糖进行治疗,同时设正常对照组(n=5)、自然恢复组(n=5)和丽珠肠乐组(n=5)。于给药7 d后处死小鼠,进行肠黏膜sIgA、血浆内毒素的检测,以及采用16S rRNA测序技术,对肠道菌群进行群落结构和多样性分析。结果给药治疗后,小鼠肠黏膜sIgA水平升高(F=3.9900,P=0.0090),血浆内毒素水平降低(F=3.8660,P=0.0100);基因测序结果显示红花多糖组和丽珠肠乐组菌群丰度与多样性均有提升,并能显著增加小鼠肠道菌群中毛螺菌属(t=2.6028,P=0.0092)、粪球菌属(t=4.5510,P<0.0001)、真杆菌属(t=2.3786,P=0.0173)、萨特菌属(t=6.2449,P<0.0001)等的相对比例,减少副拟杆菌属的含量(t=-4.6351,P<0.0001)。结论红花多糖可能通过提高小鼠肠黏膜sIgA含量,抑制血浆内毒素含量并通过增加肠道有益菌、降低致病菌、部分恢复和改善肠道菌群而发挥对微生态失调小鼠肠道菌群结构的调整作用。

Objective To observe the regulatory effect of safflower polysaccharides on intestinal microecological disorders in mice,and explore the relationship between safflower polysaccharides in improving microecological disorders and intestinal mucosal immunity,plasma endotoxin and intestinal flora.Methods The mouse model of intestinal microecological disorder was established using intragastric administration of Lincomycin hydrochloride,and then treated with safflower polysaccharides.Normal control group(n=5),natural recovery group(n=5)and Lizhu Changle group(n=5)were set up at the same time.Seven days after administration,the mice were killed for detection of intestinal mucosal sIgA and plasma endotoxin.The 16 S rRNA sequencing technique was used to analyze the community structure and diversity of intestinal flora.Results After treatment,the concentration of sIgA in intestinal mucosa of mice increased(F=3.9900,P=0.0090)while the content of plasma endotoxin decreased(F=3.8660,P=0.0100).The results of gene sequencing showed that the abundances and diversities of microflora in safflower polysaccharide group and Lizhu Changle group increased significantly,and the relative proportions of Lachnospiraceae(t=2.6028,P=0.0092),Coprococcus(t=4.5510,P<0.0001),Eubacterium(t=2.3786,P=0.0173)and Sutterella(t=6.2449,P<0.0001)significantly increased in intestinal flora of mice,while the content of Parabacteroides reduced(t=-4.6351,P<0.0001).Conclusion Carthamus tinctorius polysaccharides may play a role in regulating the structure of intestinal flora in mice by increasing the content of sIgA in intestinal mucosa,inhibiting the content of plasma endotoxin,increasing intestinal beneficial bacteria,reducing pathogenic bacteria,and partially restoring and improving intestinal flora.