肺癌合并呼吸道感染患者肺部病原菌分布及其对凋亡相关蛋白的影响

Pathogen distribution and its effect on apoptosis-associated proteins in lung cancer patients with respiratory tract infection

目的探讨肺癌合并呼吸道感染患者肺部病原菌分布及其对凋亡相关蛋白的影响,为该病的治疗提供参考。方法选择2017年3月至2019年3月我院收治的肺癌合并呼吸道感染患者62例为观察组,再选取同期我院收治的肺癌未合并呼吸道感染患者60例为对照组。分析观察组患者病原微生物分布情况,比较两组患者炎症因子[白细胞介素-6(IL-6)、白细胞介素-10(IL-10)、高迁移率族蛋白B1(HMGB1)]水平,同时比较两组患者凋亡蛋白[B淋巴细胞瘤-2蛋白(Bcl-2)、B淋巴细胞瘤-2相关X蛋白(Bax)]水平。采用Pearson相关分析法分析炎症因子与凋亡蛋白表达的相关性。结果观察组患者感染革兰阴性菌45株,占72.58%,其中以大肠埃希菌为主(27株,43.55%);感染革兰阳性菌10株,以金黄色葡萄球菌为主(6株,9.68%);感染真菌7株,占11.29%,以白假丝酵母为主(6株,9.68%)。观察组患者IL-6、IL-10、HMGB1水平均显著高于对照组(均P<0.05);观察组患者Bax水平高于对照组,Bcl-2水平低于对照组(均P<0.05);Bax与IL-6、IL-10、HMGB1水平均呈正相关(r=0.516、0.497、0.622,均P<0.001);Bcl-2与IL-6、IL-10、HMGB1水平均呈负相关(r=-0.437、-0.504、-0.433,均P<0.001)。结论肺癌合并呼吸道感染患者病原微生物以革兰阴性菌为主,病原菌可能通过炎症因子影响肺癌患者凋亡相关蛋白水平。

Objective To discuss the pathogen distribution in lung cancer patients with respiratory tract infection and its effect on apoptosis-associated proteins,and to provide a reference for the treatment.Methods Sixty-two lung cancer patients with respiratory tract infection and another 60 lung cancer patients without respiratory tract infection in our hospital from March 2017 to March 2019 were enrolled as observation group control group,respectively.The distribution of pathogenic microorganisms was analyzed among patients with respiratory tract infections.The levels of inflammatory factors[interleukin-6(IL-6),interleukin-10(IL-10),high mobility group protein B1(HMGB1)]and the expressions of apoptotic proteins[B-cell lymphoma-2(Bcl-2),Bcl2-associated X protein(Bax)]were compared between two groups.Thereafter,the correlation between inflammatory factors and apoptosis-associated protein expression was explored using Pearson correlation analysis.Results Within observation group,a total of 62 pathogens were detected,including 45 strains(72.58%)of Gram-negative bacteria dominated by Escherichia coli(27/62,43.55%),10 strains of Gram-positive bacteria(16.13%)dominated by Staphylococcus aureus(6/62,9.68%),and 7 strains of fungi(11.29%)dominated by Candida albicans(6/62,9.68%).The levels of IL-6,IL-10 and HMGB1 in observation group were significantly higher than those in control group(all P<0.05).Compared with control group,observation group had significantly higher Bax expression and lower Bcl-2 expression,with statistical difference(all P<0.05).Bax expression was positively correlated with the levels of IL-6,IL-10,and HMGB1(r=0.516,0.497,0.622;all P<0.05);Bcl-2 expression was negatively correlated with the levels of IL-6,IL-10 and HMGB1(r=-0.437,-0.504,-0.433;all P<0.05).Conclusion The main pathogenic microorganisms in patients with lung cancer complicating respiratory infections are Gram-negative bacteria,which may affect the expression of apoptosis-associated proteins in lung cancer patients via inflammatory factors.