烟草赤星病病原长柄链格孢菌快速检测胶体金试纸条的研制

Development of colloidal gold test strips for rapid detection of Alternaria longipes responsible for brown spot disease in tobacco

为实现大田期烟叶烟草赤星病的快速测定,以胶体金标记特异性识别长柄链格孢菌的单克隆抗体,利用侧流免疫层析技术构建了长柄链格孢菌快速检测试纸条。结果表明,该试纸条对目标真菌的检出限为20 ng/mL,且与烟草相关病原体无交叉反应,灵敏度高、特异性好。利用该试纸条对加标鲜烟叶进行检测,10 min内可完成检测,操作简便快速,可用于大田赤星病的快速诊断及早期预警。

To detect Alternaria longipes in tobacco fields rapidly, the monoclonal antibody against A.longipes was labelled with colloidal gold, and the rapid test strips for detecting A. longipes based on lateral flow immunoassay were developed for the first time. The results suggested that the test strips had a detection limit of 20 ng/mL and had no cross-reactivity with other tobacco-associated phytopathogens, which indicated its high sensitivity and specificity. Spiked fresh tobacco leaves were examined by using the test strips and the result could be obtained within 10 min. The colloidal gold strips are easy in operation and can be used for the rapid detection and early warning of occurrence of brown spot disease in tobacco fields.