老年胃癌患者血清中miR-483-5p表达水平及其临床意义

Serum expression levels and clinical significance of miR-483-5p in elderly patients with gastric cancer

目的探讨老年胃癌患者血清miR-483-5p相对表达水平及其临床意义。方法纳入2017年1月1日-2019年1月1日于新疆医科大学第一附属医院就诊的65例老年胃癌患者及40例健康者作为研究对象。采用RT-PCR法检测并比较2组血清中miR-483-5p相对表达水平的差异及其与临床特征之间的关系。血清miR-483-5p相对表达水平的影响因素采用logistic回归分析,利用ROC曲线分析血清miR-483-5p相对表达水平在诊断老年胃癌中的临床效能。结果老年胃癌患者血清中miR-483-5p相对表达水平(0.654±0.025)显著高于对照组(0.243±0.023,P<0.001)。侵犯层次(OR=4.123)、TNM分期(OR=3.548)、淋巴结转移(OR=4.015)均是老年胃癌患者血清miR-483-5p相对表达水平升高的危险因素。血清miR-483-5p相对表达水平诊断老年胃癌的曲线下面积为0.937(95%CI:0.848~0.966),当截断值为0.812时,诊断灵敏度和特异度分别为91.2%和95.3%。结论老年胃癌患者血清miR-483-5p相对表达水平显著升高,其表达水平与胃癌的临床特征密切相关,可作为筛查胃癌的一项生物学标志物。

Objective To investigate the relative expression levels of serum miR-483-5p and its clinical significance in elderly patients with gastric cancer.Methods A total of 65 elderly patients with gastric cancer and 40 healthy individuals were enrolled in the First Affiliated Hospital of Xinjiang Medical University between January 1,2017,and January 1,2019.The difference in the relative expression levels of serum miR-483-5p amongst the subjects was detected and compared by RT-PCR.The relationship between the relative expression levels of serum miR-483-5p and clinical characteristics of elderly patients with gastric cancer was also analysed.The influencing factors affecting the relative expression levels of serum miR-483-5p were analysed by Logistic regression analysis.The clinical efficacy of miR-483-5p in the diagnosis of gastric cancer was analysed by the receiver operating characteristic(ROC)curve.Results Compared with the control group,the relative expression levels of serum miR-483-5p in elderly patients with gastric cancer were significantly higher(P<0.001).The invasion levels(OR=4.123),TNM stage(OR=3.548)and lymph node metastasis(OR=4.015)were considered as the risk factors for the increased serum relative expression levels of miR-483-5p in elderly patients with gastric cancer.The ROC curve showed that the relative expression levels of serum miR-483-5p had a high clinical efficacy in the diagnosis of gastric cancer.Therefore,at a cutoff value of 0.812,the sensitivity and specificity were 91.2%and 95.3%,respectively.Conclusion The serum relative expression levels of miR-483-5p in elderly patients with gastric cancer are significantly high and related to the clinical characteristics.Therefore,miR-483-5p may be considered as a potential biomarker for gastric cancer screening.