血府逐瘀汤对高脂血症大鼠血液流变学及相关血清学指标的影响

Effect of Xuefu Zhuyu Decoction on hemorheology and related serological indexes in hyperlipidemia rats

目的探讨血府逐瘀汤对高脂血症大鼠血流变及相关血清学指标LDL-C、APO-A、APO-B、LP(a)的影响。方法清洁级SD大鼠40只,体重为180~220 g。随机分为空白对照组、模型对照组、血府逐瘀汤组、阿托伐他汀钙组4组,每组10只。空白对照组给予基础饲料喂饲,模型组、血府逐瘀汤组和阿托伐他汀钙组给予高脂饲料喂养制备高脂血症模型。造模时间为4周,造模结束后灌胃给药4周。血府逐瘀汤组每日给予血府逐瘀汤混悬液10 mL/(kg·d)灌胃;阿托伐他汀组每日给予阿托伐他汀混悬液10 mL/(kg·d)灌胃;模型对照组和空白对照组每日给予纯化水10 mL/(kg·d)灌胃。治疗结束后处死,采用方差分析比较各组血液流动性的化验检查(血流变)及相关血清学指标低密度脂蛋白(LDL-C)、载脂蛋白-A(APO-A)、载脂蛋白-B(APO-B)、脂蛋白[LP(a)]的差异。结果(1)血脂四项:与模型组相比,血府逐瘀汤组、阿托伐他汀钙组LDL-C、APO-A、APO-B、LP(a)水平明显下降,差异均有统计学意义(血府逐瘀汤组:t=5.01、4.19、4.62、3.28,P均<0.05;阿托伐他汀钙组:t=6.93、3.76、5.00、4.77,P均<0.05);血府逐瘀汤组与阿托伐他汀钙组的血流变及LDL-C、APO-A、APO-B、LP(a)指标比较,差异无统计学意义(P>0.05)。(2)血液流变学指标全血低切、全血高切、红细胞沉降率、红细胞压积和血小板黏附率:与模型组比较,血府逐瘀汤组和阿托伐他汀钙组差异均有统计学意义(血府逐瘀汤组:t=4.09、5.34、2.21、8.89、12.15,P均<0.001);(阿托伐他汀钙组:t=5.02、6.80、2.65、7.48、10.40,P均<0.05);血府逐瘀汤组与阿托伐他汀钙组的血流变指标比较,差异无统计学意义(P>0.05)。结论血府逐瘀汤可降低血流变及LDL-C、APO-A、APO-B、LP(a)指标,对大鼠血脂异常具有良好的调节作用。

Objective To observe the effects of Xuefu Zhuyu Decoction on blood rheology,LDL-C,APO-A,APO-B and LP(A)in hyperlipidemia rats.Methods Forty clean grade SD rats weighed 180~220 g.After successful modeling,the rats were randomly divided into 4 groups,blank control group,model control group,Xuefu Zhuyu Decoction group and atorvastatin calcium group,with 10 rats in each group.Blank control group was given basic feed,while model group,Xuefu Zhuyu Decoction group and atorvastatin calcium group were given high-fat feed to prepare hyperlipidemia model.The modeling time was 4 weeks,and the rats were given intragastric administration of drugs for 4 weeks after the modeling.Xuefu zhuyu Decoction group was given 10 mL/(kg·d)Xuefu Zhuyu Decoction suspension per day.Atorvastatin group was given 10 mL/(kg·d)atorvastatin suspension per day.Model control group and blank control group were given purified water 10 mL/(kg·d)by gavage.Rats would be killed after treatment,and variance analysis was adopted to analyze blood flow test(hemorheology)and related serological indicators of low-density lipoprotein(LDL-C),apolipoprotein-A(APO-A),apolipoprotein-B(APO-B)and lipoprotein[LP(a)]among each group.Results(1)Four items of blood lipids:Compared with model group,the levels of LDL-C,APO-A,APO-B and LP(a)in Xuefu Zhuyu Decoction group and atorvastatin calcium group were significantly decreased,and the differences were statistically significant(Xuefu Zhuyu Decoction group:t=5.01,4.19,4.62,3.28,all P<0.05;Atorvastatin calcium group:t=6.93,3.76,5.00,4.77,all P<0.05);There were no significant differences in blood rheology,LDL-C,APO-A,APO-B and LP(A)between Xuefu Zhuyu Decoction group and atorvastatin calcium group(P>0.05).(2)Hemorheological indexes:Compared with model group,the levels of whole blood low shear,whole blood high shear,erythrocyte sedimentation rate,hematocrit and platelet adhesion ratein Xuefu Zhuyu Decoction group and atorvastatin calcium group were significantly decreased,and the differences were statistically significant(Xuefu Zhuyu Decoction group:t=4.09,5.34,2.21,8.89,12.15,all P<0.05;atorvastatin calcium group:t=5.02,6.80,2.65,7.48,10.40,all P<0.05).There were no significant differences in Hemorheological indexesbetween Xuefu Zhuyu Decoction group and atorvastatin calcium group(P>0.05).Conclusion Xuefu Zhuyu Decoction can reduce hemorheology,LDL-C,APO-A,APO-B and LP(A),and has a good regulation effect on dyslipidemia in rats.