副干酪乳杆菌抑制动脉粥样硬化进展的作用及其机制

Effects and Mechanisms of Lactobacillus Paracasei on the Prevention of Atherosclerosis

【目的】探讨副干酪乳杆菌(Lpa)抑制动脉粥样硬化进展的作用及其机制。【方法】采用8周龄的雄性载脂蛋白E基因敲除(ApoE^(-/-))小鼠建立动脉粥样硬化模型,随机分为3组:①正常饲料对照组,10只ApoE^(-/-)小鼠;②模型组:10只ApoE^(-/-)小鼠,高脂高胆固醇饲料+PBS灌胃;③副干酪乳杆菌干预组:10只ApoE^(-/-)小鼠,高脂高胆固醇饲料+副干酪乳杆菌灌胃。油红O染色观察小鼠主动脉大体及主动脉窦的病理形态学变化;免疫荧光染色观察巨噬细胞脂质蓄积及胆固醇吞噬情况;Westernblot检测巨噬细胞吞噬相关蛋白B类清道夫受体(CD36)、A类清道夫受体(SR-A)的表达。【结果】副干酪乳杆菌干预组相较于模型组可以明显延缓ApoE^(-/-)小鼠动脉粥样硬化斑块形成的进展,减轻动脉血管局部的炎症反应,减少巨噬细胞脂质蓄积和巨噬细胞胆固醇吞噬,抑制巨噬细胞胆固醇吞噬相关蛋白A类清道夫受体(SR-A)的表达。【结论】副干酪乳杆菌干预可能通过抑制动脉血管局部炎症反应A类清道夫受体(SR-A)介导的巨噬细胞胆固醇吞噬,减少巨噬细胞泡沫化,从而实现其抗动脉粥样硬化的作用。

【Objective】To explore the effect and mechanism of Lactobacillus paracasei(Lpa)on the prevention of atherosclerosis.【Methods】Eight-week-old male ApoE^(-/-)mice were used to establish the atherosclerotic model,and the mice were randomly divided into three groups:(1) normal chow control group:ApoE^(-/-)+ normal chow diet(n=10);(2) model group:ApoE^(-/-)+ western diet + PBS(n=10);(3) Lactobacillus paracasei intervention group:ApoE^(-/-)+ western diet + Lactobacillus paracasei(n=10). followed by histological evaluations of atherosclerotic lesion in aorta. Immunofluorescence analysis was used to measure the cholesterol accumulation and cholesterol uptake of peritoneal macrophages. Western blot was used to measure the protein expression level of class A scavenger receptor(SR-A)and class B scavenger receptor(CD36).【Result】Intervention of Lactobacillus paracasei can significantly suppress the formation of atherosclerotic plaque in ApoE^(-/-)mice,reduce the inflammatory reaction of arterial blood vessels,inhibit the lipid accumulation and cholesterol uptake of macrophages,and reduce the protein expression of SR-A.【Conclusions】Lactobacillus paracasei may protect against atherosclerosis by preventing the inflammation of arterial blood vessels and the formation of foam cells by reducing the protein expression of SR-A.