贻贝粗多糖对环磷酰胺致小鼠免疫低下及生殖遗传毒性的保护作用

Protective Effects of Mussel Crude Polysaccharides on Immunodeficiency and Reproductive Genotoxicity Induced by Cyclophosphamide in Mice

[目的]研究贻贝粗多糖对免疫抑制小鼠的免疫功能、生殖能力和遗传稳定性的作用效果。[方法]采用环磷酰胺建立免疫抑制小鼠模型,同时灌胃木瓜蛋白酶提取的贻贝多糖进行干预,7 d后测定体重、器官指数、血常规、骨髓细胞微核率、精子致畸率。[结果]与对照组相比,在第7天模型组小鼠的体重显著下降,胸腺指数明显降低34.6%,肠指数、心脏指数分别显著升高73.4%、43.6%,小鼠的白细胞、红细胞、血小板、红细胞平均体积、红细胞压积下降,淋巴细胞、中间细胞、中性粒细胞的百分比失衡,微核率和精子畸变率分别提高了17.67千分点和7.77百分点。与模型组相比,低、高剂量组在以上指标均有不同程度的改善。[结论]一定剂量的贻贝粗多糖能改善环磷酰胺诱导免疫抑制小鼠的免疫功能,缓解环磷酰胺导致的遗传毒性和生殖毒性。

[Objective]To study the effect of immunosuppression,reproductive ability and genetic stability of mussel crude polysaccharides on cyclophosphamide-induced immunosuppression in mice.[Method]Mice were given cyclophosphamide intragastrically to establish immunosuppressive mice model,while polysaccharide from common mussel were extracted by papain was fed with mice by intragastric administration.After 7 days,effects of mussel crude polysaccharides of immunocompromised mice was studied by detecting many indices,such as body weight,organ indices,blood routine index,bone marrow cell micronucleus rate and sperm aberration rate.[Result]Compared with control group,the model group mice significantly reduced the body weight,significantly decreased the thymus index level by 34.6%,significantly increased cardiac index,the index of intestinal level by 73.4%,43.6%,respectively.The leucocytes,erythrocyte,hemoglobin,platelet,mean corpuscular volume and hematocrit of model group mice were significantly lower than those of control group.The model group mice of the percentage of lymphocytes,intermediate cells and neutrophils was inbalance,the micronucleus rate and sperm aberration rate increased by 17.67 thousandth point,7.77 percentage point and compared to the control group.Compared with model group,low and high dose groups had different degree of improvement in the above indexes.[Conclusion]A certain dose of mussel crude polysaccharide can improve the immune function of immunosuppressed mice induced by cyclophosphamide,and alleviate the genetic and reproductive toxicity caused by cyclophosphamide.