胃蛋白酶水解藏羊血清蛋白工艺研究

Study on the Process of Gastric Protease Hydrolysis and Serum Protein in Sheep

为进一步利用血液资源,以藏羊血清为原料,采用胃蛋白酶对其进行水解。以水解度为指标,研究pH、温度、加酶量3个因素对藏羊血清水解度的影响规律;在此基础上,采用Box-Benhnken响应面试验优化胃蛋白酶水解藏羊血清蛋白工艺条件。结果表明:底物浓度为5%、加酶量为6200 U时,最佳水解条件:温度为46℃、pH为2.25、酶解时间2 h,水解度为(23.41±0.23)%。

In order to make further use of blood resources,the serum of sheep was used as raw material and hydrolyzed by gastric protease.Taking hydrolysis as the index,the effect law of pH,temperature and enzyme addition on serum hydrolysis of Tibetan sheep was studied,and on this basis,the gastric protease hydrolysis process conditions of sheep serum protein were optimized by Box-Benhnken response surface test.The results showed that when the substrate concentration was 5%and the enzyme amount was 6200 U,the optimal hydrolytic conditions were as follows:temperature 46℃,pH2.25,enzymatic hydrolysis time 2 h,and the degree of hydrolysis was(23.41±0.23)%.