动物源性食品中16种β-受体激动剂高灵敏检测与膳食暴露评估

Highly Sensitive Detection and Dietary Exposure Assessment of 16β-eceptor Agonists in Animal-Derived Foods

为有效开展食品安全风险评估,对β-受体激动剂(瘦肉精)进行有效监管,本研究以超高效液相色谱-串联质谱(UPLC-MS/MS)联用法建立了动物源性食品中16种痕量β-受体激动剂的检测方法。经酶解、提取、固相萃取净化,采用多反应监测(MRM)同时捕集16种目标分子母离子-子离子离子对,7.5 min内实现高效、准确分离测定。16种目标物加标回收率为64.5%~112.3%,日间精密度0.62%~1.37%,保留时间偏差为0.010~0.042,检出限为0.05~0.84μg/kg,定量限为0.17~2.76μg/kg。应用本方法完成261批动物源性食品的安全风险监测,实现快速准确的批量筛选,发现21批样品中残留11种β-受体激动剂。依据风险监测研究结果对β-受体激动剂进行膳食暴露评估,发现当同一动物组织中同时含有多种受体激动剂,总含量超过1.08μg/kg时将增加健康风险。

In order to effectively carry out food safety risk assessment and effectively supervise for theβ-agonists,a method for the detection of 16 traceβ-agonists in animal-derived food was established by ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).After enzymatic hydrolysis,extraction,and solid-phase extraction purification,16 target molecular precursor ion-product ion ion pairs were simultaneously captured by multiple reaction monitoring(MRM),and accurate separation and determination were achieved within 7.5 minutes.The recovery rates of 16 target compounds were 64.5%~112.3%,the inter-day precision was 0.62%~1.37%,the retention time deviation was 0.010~0.042,the detection limit was 0.05-0.84μg/kg,and the quantification limit was 0.17~2.76μg/kg.Using this method,the safety risk monitoring of 261 batches of animalderived food was completed.Fast and accurate batch screening was achieved,and 11β-receptor agonists were found in 21 batches of samples.Dietary exposure to beta-agonists was assessed based on the results of risk surveillance studies.Increased health risk was found when multiple receptor agonists were present in the same animal tissue at a total level of more than 1.08μg/kg.