靶向代谢物组学检测不同日龄断奶仔猪血清中B族维生素含量

Detection of B Vitamins by Targeted Metabonomics in Serum of Weaned Piglets at Different Ages

B族维生素(VB)是维持机体健康的重要营养素,在生命体的重要代谢等方面有重要作用,被作为饲料添加剂广泛应用于畜禽养殖。由于血清成分复杂且部分VB含量极低,现有方法较难准确监测血清中VB的动态变化。本研究利用超高效液相色谱⁃串联质谱⁃多反应监测(UPLC⁃MS/MS⁃MRM)的靶向代谢组学技术成功检测了不同日龄仔猪血清中VB_(1)、VB_(2)、VB_(3)(Nicotinamide,烟酰胺)、VB_(6)(Pyridoxal,吡哆醛)、VB_(9)共5种VB含量。结果显示,随着日龄的增加,断奶仔猪血清中的VB含量均有不同程度的升高,在35日龄时断奶仔猪血清中的VB含量较28日龄升高,在63日龄时较35日龄降低并高于28日龄断奶仔猪,呈现出先升高后降低的趋势。断奶仔猪血清中烟酰胺的的含量最高,检出范围为422.95~1805.58 ng/mL;VB_(2)的含量水平次之,检出范围为182.33~561.33 ng/mL;断奶仔猪血清中VB_(1)的检出范围为23.36~72.95 ng/mL;吡哆醛的检出范围为3.48~27.90 ng/mL;断奶仔猪血清中VB_(9)的的含量最低,检出范围为5.22~22.72 ng/mL。综上,本研究利用ULPC⁃MS/MS⁃MRM技术开展靶向代谢组学分析,高效、定量地检测了猪血清样品中5种VB的含量,以期为后续畜禽的VB研究提供参考。

B vitamins(VB)are important nutrients to maintain the health and metabolism of host.It is widely used as feed additives in livestock and poultry.Due to the complex composition and extremely low content of VB in the serum,it is difficult to accurately monitor the dynamic changes of VB in serum by existing methods.In this study,five kinds of VB including VB_(1),VB_(2),VB_(3)(Nicotinamide),VB_(6)(Pyridoxal)and VB_(9) in the serum of weaned piglets at different age were successfully detected using the targeted metabolomics technique of ultra performance liquid chromatography⁃tandem mass spectrometry⁃multi⁃reaction monitoring(UPLC⁃MS/MS⁃MRM).This results showed that the content of VB in the serum of weaned piglets were increased in varying degrees with the increase of age.The content of VB in serum of weaned pigs at 35 days old were higher than that at 28 days old and at 63 days old,which showed a trend of rising first and then decreasing.The content of nicotinamide in serum of weaned piglets was the highest,and the detection range was 422.95⁃1805.58 ng/mL;The content level of VB_(2) in the serum was followed nicotinamide,with the detection range ranged from 182.33 to 561.33 ng/mL;The detection range of VB1 in the serum was from 23.36 to 72.95 ng/mL;The detection range of pyridoxal in the serum was from 3.48 to 27.90 ng/mL.The content of VB_(9) in serum was the lowest,and the detection range was from 5.22 to 22.72 ng/mL.In conclusion,5 kinds of VB in the serum of weaned piglets were detected efficiently and quantitatively by the targeted metabolomics with ULPC⁃MS/MS⁃MRM technology in this study,which provided a reference for subsequent research on VB in livestock and poultry.