基于网络药理学探究人参-三七药对改善无症状性心肌缺血作用机制

To Explore the Mechanism of Radix Ginseng-Radix Notoginseng on Improving Asymptomatic Myocardial Ischemia Based on Network Pharmacology

目的:借助网络药理学研究人参-三七药对治疗无症状性心肌缺血的作用机制。方法:借助中药系统药理学数据库与分析平台(TCMSP),以“人参”“三七”为检索词获取药物成分及作用靶点,运用UniProt对基因靶点标准化处理,利用Cytoscape 3.7.2软件构建成分-靶点网络图;以GeneCards平台挖掘SMI作用靶点;使用韦恩图得到药物-疾病交集靶点。基于STRING数据库构建蛋白质-蛋白质相互作用(PPI)网络,进行可视化分析,筛选核心靶点。利用Metascape数据库及微生信在线绘图平台对药物-疾病核心靶点进行基因本体(GO)富集分析和京都基因和基因组百科全书(KEGG)富集分析。结果:共得到22个活性成分,4个共有成分,125个作用靶点,1242个SMI相关靶点,94个交集靶点,39个核心靶点。主要的活性成分包括山柰酚、槲皮素、β-谷固醇等,后二者为药对共有成分;核心靶点包括IL6、VEGFA、TNF、TP53等。GO富集分析发现积极调控细胞迁移、细胞对脂质的反应、肌肉细胞增殖、活性氧代谢过程、细胞对氧化应激的反应等生物过程;KGEE通路分析得到糖尿病并发症中的AGE-RAGE信号通路、癌症的途径、IL-17信号通路、HIF-1信号通路、松弛素信号通路等通路。结论:本研究表明人参-三七药对可通过参与上述生物过程调控信号通路发挥抗炎、抗细胞凋亡、改善血管通透性等作用,进一步说明了人参-三七治疗SMI的通路较多,机制较复杂。

Objective:To study the mechanism of Radix Ginseng-Radix Notoginseng in the treatment of asymptomatic myocardial ischemia(SMI)by network pharmacology.Methods:With the help of TCMSP database,we used“Radix Ginseng”and“Radix Notoginseng”as search terms to obtain drug components and targets,and UniProt to standardize gene targets.We used Cytoscape 3.7.2 software to construct component-target network diagrams;used GeneCards platform to mine SMI targets;used Venn diagrams to obtain drug-disease intersection targets.A protein interaction network(PPI)was constructed based on the STRING database for visual analysis and screening of core targets.GO enrichment analysis and KEGG pathway analysis of core drug-disease targets were performed using Metascape database and WeChat online mapping platform.Results:A total of 22 active ingredients were obtained by analysis,4 common ingredients,125 targets,1242 SMI related targets,94 intersection targets,39 core targets.Among the main active ingredients are kaempferol,quercetin,β-guillotine,The latter two are the common components of the drug;core targets include IL6,VEGFA,TNF,TP53 and etc.GO enrichment analysis found that active regulation of cell migration,cell response to lipid,muscle cell appreciation,active oxygen metabolism process,cell response to oxidative stress and other biological processes;KGEE pathway analysis obtained AGE-RAGE signaling pathway,cancer pathway,IL-17 signaling pathway,HIF-1 signaling pathway,relaxin signaling pathway and so on in diabetic complications.Conclusion:In this study,Radix Ginseng-Radix Notoginseng drugs can play anti-inflammatory,anti-apoptosis and improve vascular permeability through regulating signaling pathway,further explained that Radix Ginseng-Radix Notoginseng treatment SMI more pathways,the mechanism is more complex.