长链非编码RNA LINC00319在肝细胞癌中表达上调并促进肝癌细胞迁移和侵袭

Observation of the high-expression of long-chain non-coding RNA LINC00319 and promotion of cell migration and invasion in hepatocellular carcinoma

目的:探讨LINC00319在肝细胞癌(HCC)组织中的表达、临床意义及可能的分子机制。方法:实时定量PCR检测LINC00319在HCC及癌旁组织中的表达水平,并分析LINC00319表达水平与临床病理特征的关系及生存率。Transwell实验检测LINC00319对HCC细胞的侵袭及迁移作用,Western blotting法检测LINC00319对HCC细胞EMT的影响。结果:LINC00319在肝癌组织中表达水平明显高于相应癌旁组织(P<0.01);肝癌组织LINC00319高表达与TNM分期及血管侵犯相关(P<0.01和P<0.05);高表达LINC00319病人5年生存率明显高于低表达组(P<0.01);LINC00319下调可显著抑制HCC细胞的侵袭及迁移(P<0.01);LINC00319下调抑制HCC细胞EMT的发生(P<0.01)。结论:LINC00319在肝癌组织中表达升高,其高表达与肝癌恶性临床病理特征有关,LINC00319能够通过调控HCC细胞EMT促进其侵袭和迁移。

Objective:To investigate the level and clinical significance of LINC00319 expression in human hepatocellular carcinoma(HCC)and analyze the potential underlying mechanisms.Methods:The LINC00319 expression level in HCC was checked by real time quantitative PCR method,and the relationship among LINC00319 expression level,clinical pathological features and survival rate were analyzed.Transwell assay was performed to measure the invasion and migration of effects of LINC00319 on HCC cells.The expression of EMT-related protein was determined by Western blotting.Results:The expression level of LINC00319 in HCC tissues was significantly higher than that in the adjacent normal tissues(P<0.01),and it was significantly associated with TNM stage and vascular infiltration(P<0.01 and P<0.05).Patients in the higher LINC00319 group had a worse 5-year survival than those in the lower LINC00319 group(P<0.01).Down-expression of LINC00319 significantly inhibited the invasion and migration of HCC cells(P<0.01).Down-expression of LINC00319 inhibited the EMT of HCC(P<0.01).Conclusions:LINC00319 is up-regulated in HCC tissues.Its high expression is correlated with the malignant clinical pathological features.LINC00319 high-expression regulates EMT and subsequently promotes invasion and migration of HCC cells.