菊粉、抗性淀粉RS3及其复合物对小鼠脂肪代谢及肠道菌群的影响

Effects of inulin,resistant starch RS3 and their complexes on blood lipid levels and intestinal flora in mice

【目的】探讨菊粉、抗性淀粉RS3及其复合物的不同饮食干预对小鼠体重和肠道菌群的影响。【方法】64只C57BL/6小鼠随机分为低脂对照组、低脂菊粉组、低脂抗性淀粉RS3组、低脂复合组和高脂对照组、高脂菊粉组、高脂抗性淀粉RS3组、高脂复合组等8组,分别喂养。每周称量小鼠体重和收集新鲜粪便,饮食干预直至高脂对照组比低脂对照组体重高14%以上。小鼠过夜禁食后解剖,称量肝脏、附睾脂肪,采集结肠内容物进行肠道菌群16S rRNA编码基因的扩增子测序分析。【结果】在低脂饮食小鼠中,菊粉和抗性淀粉RS3复合干预可引起体重增加值的显著降低;在高脂饮食小鼠中,单独进行菊粉干预和两者复合干预都能够引起体重增加值的显著降低,2种饮食干预之间无明显差异。高脂饮食中菊粉、抗性淀粉RS3、复合干预组分别以拟杆菌属、双歧杆菌属、Alloprevotella菌属为主要差异细菌;低脂饮食中菊粉、抗性淀粉RS3、复合干预组分别以Coriobacteriaceae_UCG_002、拟杆菌属、螺杆菌属为主要差异细菌。【结论】菊粉和抗性淀粉RS3饮食干预可显著降低C57BL/6小鼠体重增加幅度,改变肠道菌群结构,同时显示出高脂、低脂饮食差异。菊粉和抗性淀粉RS3可能通过改变肠道菌群结构从而发挥降低体重作用,促进脂肪代谢。

[Objective]To explore the effects of different dietary induction models of inulin,resistant starch RS3 and their complexes on the body weight and intestinal flora in mice.[Methods]A total of 64 C57 BL/6 mice were randomly divided into low-fat control group,low-fat inulin group,low-fat resistant starch RS3 group,low-fat composite group and high-fat control group,high-fat inulin group,high-fat resistant starch RS3 group and high-fat composite group for dietary intervention.The mice were weighed and fresh feces were collected weekly.Diet intervention was continued until the weight of the high-fat control group was more than 14%higher than that of the low-fat control group.The mice were then sacrificed after overnight fasting.Liver and epididymal fat were weighed,and the colon contents were collected for 16 S amplicon sequencing analysis.[Results]In low-fat diet fed mice,the combined induction of inulin and resistant starch RS3 caused a significant decrease in body weight gain.In high-fat diet fed mice,inulin alone and the combined induction both caused a significant reduction in weight gain,and there was no significant difference between the two methods.In the high-fat diet groups,inulin,resistant starch RS3,and the compound could be distinguished by Bacteroides,Bifidobacterium and Alloprevotella respectively.In the low-fat diet groups,inulin,resistant starch RS3,and the composite groups could be distinguished by Coriobacteriaceae_UCG_002,Bacteroides and Helicobacter,respectively.[Conclusion]Inulin and resistant starch RS3 diet induction can significantly reduce the weight gain of C57 BL/6 mice,change the structure of intestinal flora,and show the difference between high-fat and low-fat diets.Inulin and resistant starch RS3 may reduce body weight and promote fat metabolism by changing the structure of intestinal flora.