柚皮苷-PLGA微球的制备及表征

Preparation and Characterization of Naringin-PLGA Microspheres

目的:制备柚皮苷-PLGA缓释微球,并对其性能进行体外评价。为促骨生长类药物的长效制剂的设计与研发奠定基础。方法:以聚乙烯醇(PVA)、聚乳酸-羟基乙酸共聚物(PLGA)为复合载体材料,采用乳化溶剂挥发法制备柚皮苷-PLGA微球,以微球外观形态、包封率为主要评价指标,单因素投料比(1︰5、1︰10、1︰15)、转速(1500 r/min、2000 r/min、3000 r/min)以及PVA(1%、2%、3%)考察法筛选处方。扫描电镜(SEM)观察外观形态,对最佳处方制备所得微球进行物相表征。结果:投药比为1︰10、转速为1500 r/min、1%PVA浓度制备的微球载药量和包封率较好,载药量为4.56%~12.54%,包封率为34.66%~95.30%。结论:该制备方法获得柚皮苷-PLGA微球,制备方法准确可靠。

Object: Naringin-PLGA sustained-release microspheres were prepared and their properties were evaluated in vitro. It lays a foundation for the design and development of long-acting preparations of bone growth promoting drugs. Methods: Naringin-PLGA microspheres were prepared by emulsifying solvent volatilization with polyvinyl alcohol and polylactic acid-hydroxyacetic acid copolymer as the composite carrier. The appearance and encapsulation efficiency of the microspheres were the main evaluation indexes. The prescriptions were screened by single factor feeding ratio(1︰5, 1︰10, 1︰15), rotational speed(1500 r/min,2000 r/min, 3000 r/min) and PVA(1 %, 2 %, 3 %). The morphology was observed by SEM, and the microspheres was characterized. Results: The drug loading rate and encapsulation efficiency of the microspheres prepared at the dosage ratio of 1︰10, rotating speed of 1500 r/min and concentration of 1 % PVA were better, the drug loading rate was 4.56 %~12.54 %. Conclusion: The preparation method obtains naringin-PLGA microspheres, and the preparation method is accurate and reliable.