硝基取代钌配合物-适配体荧光探针检测汞离子

Detection of Hg^(2+) by Nitro-substituted Ruthenium Complex-aptamer Luminescent Probe

生物体内汞离子过量会对神经系统和正常的代谢活动造成极大的伤害。因此有必要发展新的方法用于汞离子的高灵敏、高选择性检测。本研究采用钌配合物-适配体探针体系,利用钌配合物可选择性识别G-四链体DNA以及核酸适配体中T碱基与汞离子特异性结合的特性,建立了一种荧光检测汞离子的方法。当无汞离子时,核酸适配体形成G-四链体结构;有汞离子时,则形成双链结构。在优化条件下,利用适配体与汞离子的特异性结合,该探针可实现对汞离子的荧光检测。探针荧光强度与汞浓度的线性范围为2~150μmol/L,检测限为0.86μmol/L。该方法为钌配合物-适配体复合体系用于汞离子的定量检测提供了新途径。

Excessive Hg^(2+) in organisms can cause great harm to the nervous system and normal metabolic activities.It is necessary to develop new methods for sensitive and selective detection of Hg^(2+).In this study,ruthenium complex-aptamer luminescent probe has been used to develop a new method to detect Hg^(2+) based on the selective recognition of G-quadruplex DNA by ruthenium complex and the specific binding of the aptamer to Hg^(2+).The aptamers formed G-quadruplex structure in the absence of mercury ion,while after binding to Hg^(2+),double-chain structure appeared.Under the optimized conditions,the luminescent detection of Hg^(2+) can be performed using the specific binding of the aptamer to Hg^(2+).The linear range of luminescence intensity and Hg^(2+) concentration was 2~150μmol/L and the detection limit was 0.86μmol/L.This method provides a new approach for the ruthenium complex-aptamer system for the quantitative detection of Hg^(2+).