基于胸腺嘧啶碱基错配和金纳米棒比色型传感器对汞离子的可视化检测

Construction of Colorimetric Sensors for Visual Detection of Mercury Ions Based on Bases Mismatches of Thymine DNA and Gold Nanorod

基于未修饰的金纳米棒(AuNR)和富含胸腺嘧啶的单链DNA (T-DNA)序列,本论文构建了一种比色传感器,并实现了对汞离子的可视化检测。结果表明,当NaCl浓度为200μM,NaCl反应时间为8 min,T-DNA浓度为600 nM,Hg^(2+)孵育时间为5 min时,开发的传感器对Hg^(2+)表现出高选择性和灵敏度,线性范围和检测限分别为1~200 nM和0.62 nM,相关系数为0.995,添加回收率为96.37%~102.14%,构建的比色传感器可对5 nM及以上浓度的Hg^(2+)实现可视化检测。

In this paper, we constructed a colorimetric sensor and realized the visual detection of mercury ions based on unmodified gold nanorods(AuNR) and thymine-rich single-strand DNA(T-DNA) sequences. The results showed that when the NaCl concentration was 200 μM, the reaction time of NaCl was 8 min, the T-DNA concentration was 600 nM, and the incubation time of Hg^(2+) was 5 min, the developed sensor showed high selectivity and sensitivity to Hg^(2+). The linear range and the detection limit were 1~200 n M and 0.62 nM, with the correlation coefficient of 0.995. The recovery rate was between 96.37 % and 102.14 %. The constructed colorimetric sensor can realize the visual detection of Hg^(2+) when the concentration of Hg^(2+) was 5 nM or above.