杉木NAC基因家族基因的鉴定及生物信息学分析

Identification and bioinformatics analysis of Chinese fir NAC gene family

【目的】为了完善杉木NAC基因家族的生信机制以及探索基因潜在功能,促进杉木基因改良育种与提高生产效率。【方法】本研究从搜集到的杉木转录组测序数据库中筛选鉴定得到杉木NAC基因,并对其进行生物信息学分析,具体包括蛋白的理化性质、结构域预测、基因进化树分析和cln-miR164靶基因的预测分析等。【结果】杉木NAC转录因子家族含有45个成员,将其命名为Cl-NAC1~Cl-NAC45,预测所得CDS序列长度在156~3033 bp之间,由51~1010个氨基酸残基构成,结构域分析得出Cl-NACs蛋白除了长度不足的序列,所有的NAC蛋白都具有A、B、C、D、E 5个亚结构域,并且位置一致。保守域预测可得不同亚家族7个保守结构域的位置和长度有所不同。大部分Cl-NACs基因与挪威云杉NAC基因序列相似度明显大于拟南芥。通过进化树分析,不同Cl-NACs蛋白分布在10个亚家族中ATAF亚家族中的Cl-NAC45可能与逆境胁迫相关,NAM亚家族中的Cl-NAC1、Cl-NAC33、Cl-NAC34和Cl-NAC35基因都有可能具有调节杉木生长发育的作用;此外通过psRNATarget预测Cl-NAC33和Cl-NAC35为cln-miR164的靶基因,可能受其调控,参与非生物胁迫响应途径。【结论】杉木NAC基因鉴定获得45个,共可分为10个亚家族,不同的亚家族具有不同的理化性质、蛋白质结构、进化模式。对不同亚家族的基因功能进行初步推测,上述7个基因可能为杉木生长发育中的关键基因,需进一步研究验证其功能,为后续研究Cl-NACs基因对杉木生长发育、抗逆性功能奠定了基础。

【Objective】This study was designed to improve the bioinformatics analysis mechanism and explore the potential functions of the Chinese fir NAC gene family,and promote the genetic improvement of fir breeding and its production efficiency.【Method】The NAC genes of Chinese fir were screened and identified from the collected Chinese fir transcriptome sequencing database,and the bioinformatics analysis was performed,including the prediction of physical and chemical properties and domains of proteins,gene evolution tree analysis,and the prediction of cln-miR164 target genes.【Result】The NAC transcription factor family of Cunninghamia lanceolata contained 45 members,which were named Cl-NAC1-Cl-NAC45.The predicted CDS sequences were between 156 and 3033 bp,consisting of 51 to 1010 amino acid residues.The domain analysis showed that all the NAC proteins had five subdomains including A,B,C,D,and E and the positions were the same.The encoding proteins were grouped into 10 subfamilies.Conserved domain prediction revealed that the position and length of the 7 conserved domains in different subfamilies were varied.According to phylogenetic tree analysis,Cl-NAC45 from the ATAF subfamily may be related to stress responses.The four genes Cl-NAC1,Cl-NAC33,Cl-NAC34 and Cl-NAC35 from the NAM subfamily may play roles in the regulation of the growth and development of Chinese fir.By using psRNATarget,it is predicted that Cl-NAC33 and Cl-NAC35 may be target genes of cln-miR164,which were involved in the response to abiotic stress.【Conclusion】45 Chinese fir NAC genes were identified,which can be divided into 10 subfamilies.Proteins from different subfamilies exhibited different physical and chemical properties,structures and evolutionary patterns.Preliminary speculations on the gene functions of different subfamilies were made.The 7 genes mentioned above may be key genes involved in the growth and development of Chinese fir,and further studies are needed to verify their functions,in order to lay a foundation for the follow-up study of Cl-NACs gene functions on the growth,development and stress resistance of Chinese fir.