植物提取物对浅黄假单胞菌生物被膜的作用

Effect of Plant Extract on Pseudomonas luteola Biofilm

本文研究具有抑菌效果的药食同源植物提取物对冷却肉中关键腐败菌——假单胞菌生物被膜的抑制、清除效果。从市售冷却肉中分离一株具有较强生物被膜形成能力的假单胞菌,经鉴定为浅黄假单胞菌8-4。采用结晶紫染色法观察蒲公英、金银花、丁香、肉桂、薄荷、桑叶等6种提取物对浅黄假单胞菌生物被膜的抑制和清除作用。结果表明:金银花提取物和蒲公英提取物对浅黄假单胞菌的抑制和清除效果最佳。为进一步分析其对生物被膜的作用机制,将提取物的共同功效性成分绿原酸作用于生物被膜,测定绿原酸对浅黄假单胞菌生物被膜的抑制、清除作用及对生物被膜代谢活性的影响。采用激光共聚焦扫描电镜观察绿原酸对生物被膜微观结构的变化。结果表明:绿原酸对浅黄假单胞菌的抑制和清除作用效果显著,在绿原酸作用下生物被膜的代谢活性降低。在高质量浓度绿原酸作用下菌体无法聚集黏连形成生物被膜,而低质量浓度作用下的菌体虽能形成生物被膜,但结构立体性较低。结论:植物提取物中的绿原酸通过抑制菌体生长而抑制生物被膜形成,通过弱化成熟生物被膜立体结构而清除生物被膜。

In order to research the inhibitory effect and mechanism of plant extracts on the biofilm of Pseudomonas luteola.The effects of 6 kinds of extracts,such as dandelion,honeysuckle,clove,cinnamon,mint and mulberry leaf,on the biofilm formation of Pseudomonas luteola were measured.The clearance rate and inhibition rate of biofilm were calculated.The results showed that the extracts of dandelion and honeysuckle,which contains the same functional components-chlorogenic acid(CA),has the best inhibitory and scavenging effects on the biofilm of Pseudomonas luteola.Under the action of chlorogenic acid,the amount of biofilm was determined by crystal violet method,the metabolic activity of biofilm was determined by MTT method,and the apparent morphology of biofilm was analyzed by laser confocal scanning electron microscopy.CA had a significant inhibitory and scavenging effect on Pseudomonas luteola.The metabolic activity of biofilm was reduced under the action of CA.High concentrations of CA prevented the bacteria from conglutinating to form biofilm.The bacteria could form biofilm at low concentrations,but the stereoscopic property of the structure was low.Conclusion:CA from the plant extract could inhibit the formation of biofilm by inhibiting the growth of bacteria,weake the three-dimensional structure of mature biofilm,and have the ability to clear biofilm.