西兰花种子油中甘油三酯和脂肪酸组成分析

Triacylglycerols and Fatty Acid Compositions Analyses of Broccoli Seed Oil

分别使用超高效超临界流体色谱-四极杆飞行时间质谱联用仪和气相色谱-质谱联用仪鉴定西兰花种子油中甘油三酯(TAG)与脂肪酸组成。根据高分辨质谱一级质谱的母离子和二级质谱的碎片离子信息从西兰花种子油中鉴定出43种甘油三酯,采用峰面积归一化法对其在种子油中的相对含量进行表征。结果表明:鉴定的43种甘油三酯中含有13对同分异构体,1-芥酸-2-亚油酸-3-芥酸酯(ELE)、1-芥酸-2-亚麻酸-3-芥酸酯(ELnE)和1-花生烯酸-2-亚麻酸-3-芥酸酯(GLnE)是西兰花种子油中含量最高的3种甘油三酯,15种含有芥酸侧链的甘油三酯占总甘油三酯的77.99%。脂肪酸组成测定表明:芥酸在西兰花种子油(53.13%)中含量最高,其次为油酸(20.92%)和亚油酸(11.82%)。研究结果增进了对西兰花种子油中化学物质组成的了解,为该种子油的综合开发利用提供参考。

Triacylglycerols(TAGs)and free fatty acids compositions of broccoli seed oil were analyzed using ultra-performance convergence chromatography(UPC2)combined with quadrupole time-of-flight mass spectrometry(Q-TOF MS)and gas chromatography(GC)combined with mass spectrometry(MS).A total of 43 different TAGs were characterized based on their Q-TOF MS accurate molecular weight and MS2 fragment ion profiles in the broccoli seed oil.There were 13 pairs of isomers in 43 TAGs.ELE,ELnE and GLnE were the three major TAGs.Among all TAGs,15 TAGs contained erucin,contributing about 77.99%of the total TAGs.For the fatty acid compositions,erucic acid was the greatest fatty acid in broccoli seed oil,followed by oleic acid and linoleic acid.The chemical compositions of TAGs and fatty acids could promote the understanding about the chemical profiles of broccoli seed oil,thus improve the potential availabilities of developing function foods with broccoli seed oil in the future.