结直肠癌周期蛋白E和生存素的表达及临床意义

Expression and Clinical Significance of Cyclin E and Survivin in Colorectal Cancer

目的探究结直肠癌中周期蛋白E和生存素的表达及临床意义。方法选取2018年1月至2021年9月于安徽医科大学附属第三医院进行手术切除治疗的60例结直肠癌患者的结直肠癌组织,对选取的标本进行分级、分期等,选取同期进行结直肠外科手术的30例非结直肠癌患者的正常结直肠组织作为对照。采用免疫组织化学法检测周期蛋白E和生存素的表达,同时分析周期蛋白E和生存素阳性表达强度与相关临床病理因素的相关性。结果结直肠癌组织组中周期蛋白E和生存素的阳性表达率高于正常结直肠组织[75.0%(45/60)比3.3%(1/30)、50.0%(30/60)比0](χ^(2)=30.077,P<0.001;χ^(2)=16.000,P<0.001);不同性别、组织分化、肿瘤浸润位置周期蛋白E和生存素阳性表达率比较差异均无统计学意义(P>0.05);年龄≥50岁、Dukes分期为Ⅲ~Ⅳ期、发生淋巴结转移者周期蛋白E和生存素阳性表达率均较高(P<0.05);周期蛋白E和生存素阳性表达强度与Dukes分期和淋巴结转移均呈正相关(P<0.01)。结论周期蛋白E和生存素表达与结直肠癌的Dukes分期、淋巴结转移存在密切关系,有助于评估结直肠癌患者临床状态,为疾病治疗提供参考依据。

Objective To explore the clinical significance of cyclin E and survivin in colorectal cancer.Methods Colorectal cancer tissues of 60 patients with colorectal cancer surgically removed in the Third Affiliated Hospital of Anhui Medical University from Jan.2018 to Sep.2021 were collected.The selected specimens were graded and staged,normal colorectal tissues of 30 patients with non-colrectal cancer who underwent colorectal surgery at the same time were selected as controls.The expressions of cyclin E and survivin were detected by immunohistochemistry.The correlation between the positive expression degree of cyclin E and survivin and related clinicopathological factors was analyzed.Results The positive expression rates of cyclin E and survivin in the colorectal cancer tissues were higher than those in the normal colorectal tissues[75.0%(45/60)vs 3.3%(1/30),50.0%(30/60)vs 0](χ^(2)=30.077,P<0.001;χ^(2)=16.000,P<0.001).There was no significant difference in the positive expression rates of cyclin E and survivin between different genders,tissue differentiation degrees and tumor invasion locations(P>0.05).The positive expression rates of cyclin E and survivin were higher in patients with age≥50 years,Dukes stageⅢ-Ⅳ,lymph node metastasis(P<0.05).The positive expression of cyclin E and survivin were positively correlated with Dukes stage and lymph node metastasis(P<0.05).Conclusion The expression of cyclin E and survivin is closely related to the Dukes stage and lymphatic metastasis of colorectal cancer,which is helpful to assess the clinical status of the patients with colorectal cancer and provide reference basis for the treatment.