提取条件对蜂花粉粗多糖抗氧化能力的影响

Effect of Extraction Conditions on Antioxidant Activity of Bee Pollen Polysaccharides

为探究不同提取方式对蜂花粉粗多糖抗氧化活性的影响,采用热水浸提法提取蜂花粉粗多糖,研究了温度及乙醇体积分数对3种花粉中蜂花粉粗多糖提取率的影响;并根据邻苯三酚自氧化原理和芬顿原理检测了蜂花粉粗多糖对超氧阴离子和羟自由基的清除效果;最后,以经丙烯醛处理的大黄鱼肉为模型,通过测定蛋白质羰基质量摩尔浓度研究了蜂花粉粗多糖对大黄鱼肌原纤维蛋白质氧化的影响。结果表明:80℃提取的茶花花粉粗多糖对超氧阴离子的抗氧化活性最显著,乙醇体积分数对3种花粉粗多糖对超氧阴离子的清除效果差别显著,而对羟自由基的清除效果不显著。80℃、乙醇体积分数为95%时所提取的3种蜂花粉粗多糖均能使丙烯醛处理的大黄鱼肉肌原纤维蛋白中羰基质量摩尔浓度显著下降而总巯基质量摩尔浓度上升,且以野菊花粉粗多糖作用效果最佳。因此,蜂花粉粗多糖的抗氧化活性受提取温度和乙醇体积分数的影响,且蜂花粉粗多糖能够抑制蛋白质氧化,其中以野菊花粉粗多糖抗氧化效果最佳。

Hot water extraction was used to extract bee pollen crude polysaccharides to investigate the effect of different extraction methods on the antioxidant activities of bee pollen polysaccharides.The effect of temperature and ethanol volume fraction on the extraction rate of bee pollen polysaccharide from three kinds of pollenwere studied. The scavenging effect of bee pollen crude polysaccharides towards superoxide and hydroxyl radical was detected under pyrogallol autoxidation and fenton oxidation, respectively. At last, the effect of pollen polysaccharides on myofibrillar protein oxidation was explored by measuring the content of protein carbonyl group(molar concentration) in the flesh of acrolein-treated large yellow croaker(Pseudosciaenacrocea). The results indicated that the antioxidant effect of camellia crude polysaccharides extracted at 80℃ was most significant against superoxide radicals. The scavenging effect of ethanol volume fraction on superoxide free radicals of three kinds of pollen polysaccharides was significant, but the scavenging effect onhydroxyl radicals was insignificant. Pollen polysaccharides extracted at 80 ℃ and 95%ethanol concentration significantly reduced the carbonyl content and increased total sulfhydryl content in the large yellow croaker myofibrillar protein treated with acrolein. The effect of crude polysaccharides from wild chrysanthemum pollen was the best. It was concluded that the antioxidant activity of bee pollen was affected by extraction temperature and ethanol volume fraction, and bee pollen polysaccharides couldinhibit protein oxidation, among which wild chrysanthemum pollen polysaccharides showed the best antioxidant effect.