玫瑰红枣牛初乳冻干粉的研制及其抗氧化活性研究

Preparation and Antioxidant Activity of Lyophilized Powder of Rose Jujube and Bovine Colostrum

以玫瑰花预处理沉淀物、红枣浓缩汁、低聚果糖和赤藓糖醇为主要研究对象,以感官得分为评价指标,通过单因素试验和正交试验方法确定玫瑰红枣牛初乳冻干粉的最佳配方,并进一步测定玫瑰红枣牛初乳冻干粉对DPPH自由基、超氧阴离子自由基和对羟基自由基的清除率,评价玫瑰红枣牛初乳冻干粉抗氧化活性。结果表明,玫瑰花预处理沉淀物的最佳添加量为2.5%,红枣浓缩汁的最佳添加量为5%,低聚果糖的最佳添加量为7.5%,赤藓糖醇的最佳添加量为17.5%,因此,玫瑰红枣牛初乳冻干粉的最佳配方为玫瑰花174.8%,红枣浓缩汁5%,N-乙酰神经氨酸5%,透明质酸钠2%,胶原蛋白肽40%,弹性蛋白0.2%,雨生红球藻5%,低聚果糖7.5%,复配营养强化剂(维生素C+维生素E+烟酸+麦芽糊精)1%,赤藓糖醇17.5%,生鲜牛初乳138.8%。体外抗氧化试验得到玫瑰红枣牛初乳冻干粉对O_(2)^(-)、DPPH和·OH的清除率分别为77.41%、87.68%和57.13%,显著高于对照组(P<0.05),可见玫瑰红枣牛初乳冻干粉具有抗氧化活性,为深度开发牛初乳冻干粉新方向提供参考。

Taking pre-treated sediment of rose,concentrated jujube juice,fructose-oligosaccharides and erythritol as the main research objects,single factor experiment and orthogonal experiment method were used to determine the best formula of freeze-dried powder of rose jujube bovine colostrum with sensory score as evaluation index.Furthermore,the scavenging rates of DPPH free radical,superoxide anion free radical and hydroxyl free radical were determined,and the antioxidant activity of the freeze-dried powder was evaluated.The results showed that the optimum dosage of pre-treated sediment of rose was 2.5%,that of concentrated jujube juice was 5%,that of fructose-oligosaccharides was 7.5%,and that of erythritol was 17.5%.Therefore,the optimum dosage of freeze-dried powder of rose jujube bovine colostrum was 174.8%,that of concentrated jujube juice was 5%.N-acetylneuraminic acid 5%,sodium hyaluronate 2%,collagen peptide 40%,elastic protein 0.2%,haematochromochlorella 5%,oligosaccharides 7.5%,nutrient fortifier(vitamin C+vitamin E+niacin+maltodextrin)1%,erythritol 17.5%,fresh bovine colostrum 138.8%.The scavenging rates of O_(2)^(-),DPPH and·OH in vitro experiments were 77.41%,87.68%and 57.13%,respectively,which were significantly higher than those in the control group(P<0.05).It can be seen that the freeze-dried powder has antioxidant activity,providing reference for further development of new direction of freeze-dried powder of bovine colostrum.