高效液相色谱法测定染发产品中8种染发剂

Determination of Eight Hair Dyes in Hair Dye Products by High Performance Liquid Chromatography

建立了高效液相色谱法同时测定8种染发剂的检测方法。样品采用甲醇超声提取,使用Purospher;STARLPRP-18 endcapped色谱柱分离,以乙腈-0.02 mol/L乙酸铵为流动相,检测波长HC黄2为230 nm,分散蓝1为240 nm,HC红3号、分散紫1和HC橙1为250 nm,HC红1号为270 nm,HC黄4为420 nm,分散黑9为450 nm。该方法在1.0~100.0μg/mL范围内线性良好,相关系数均高于0.999,检出限为0.60~0.95 mg/kg,加标回收率为84.2%~108.4%,相对标准偏差为1.3%~4.5%。该方法简便快速、结果准确,能够满足染发产品中8种染发剂的分析要求。

A high performance liquid chromatographic(HPLC)method for simultaneous determination of eight hair dyes was established.The samples were extracted with methanol by ultrasonic extraction and separated on Purospher;STAR LP RP-18 endcapped column with mobile phase of acetonitrile and 0.02 mol/L ammonium acetate.The detection wavelength was 230 nm for HC yellow 2,240 nm for disperse blue 1,250 nm for HC red 3,disperse violet 1 and HC orange 1,270 nm for HC red 1,420 nm for HC yellow 4 and 450 nm for disperse black 9.The linear range of the method was 1.0-100.0μg/m L with the correlation coefficients higher than 0.999.The detection limits were 0.60-0.95 mg/kg.The recoveries were in the range of 84.2%-108.4%with the relative standard deviations of 1.3%-4.5%.The method is simple,quick and accurate,which can meet the analysis requirements of eight hair dyes in hair dye products.