非洲辣木组培快繁再生技术体系的建立

Establishment of Regeneration System for Tissue Culture and Rapid Propagation of Moringa stenopetala

为优化非洲辣木(Moringa stenopetala)组培快繁再生技术,提升种苗品质,以非洲辣木无菌苗为试验材料,研究培养基和植物生长调节剂对非洲辣木不同生长阶段的影响。结果表明,非洲辣木种子经清水浸泡30 min+75%酒精消毒1 min+0.1%升汞消毒10 min处理后,接种于MS培养基上,种子萌芽率达81.11%;愈伤组织诱导的最适培养基为MS+6-BA1.0 mg/L+KT 1.0 mg/L,诱导率为89.89%;初代芽在MS+6-BA 0.6 mg/L+KT 0.3 mg/L培养基上进行继代增殖培养,增殖系数可达4.62;在1/2 MS+NAA 1.0 mg/L+IBA 1.0 mg/L培养基上进行生根培养,生根数为5.92,根长为1.94 cm,生根率为96.67%;以泥炭土为移栽基质,移栽成活率最高(94.44%)。通过该组培快繁再生技术体系,非洲辣木诱导率和增殖系数高,生根效果好,苗木生长快速。

In order to optimize tissue culture and regeneration technology and improve seeding quality,Moringa stenopetala non-bacterial seedlings were used as experimental materials to study effects of medium and plant growth regulator on growth of M.stenopetala at different growth stages.Results showed that M.stenopetala seeds were treated by water soaking for 30 min,75%alcohol sterilizing for 1 min,0.1%mercury bichloride sterilizing for 10 min,and were inoculated on MS medium,germination rate of seed reached 81.11%.The optimal medium for callus induction was MS+6-BA 1.0 mg/L+KT 1.0 mg/L,and induction rate was 89.89%.The optimal medium for subculture proliferation of primary bud was MS+6-BA 0.6 mg/L+KT 0.3 mg/L,and proliferation coefficient was 4.62.The optimal medium for rooting was 1/2 MS+NAA 1.0 mg/L+IBA 1.0 mg/L with number of roots 5.92,root length 1.94 cm,and rooting rate 96.67%.Peat soil was selected as transplanting substrate with the highest transplanting survival rate(94.44%).By the system,M.stenopetala had high induction rate and proliferation coefficient,good rooting effect and rapid growth of seedlings.