基于数据库分析MECOM在卵巢癌中的表达和临床意义

The Expression and Clinical Significance of MECOM in Ovarian Cancer:An Analysis Based on Database

目的:探究MECOM在卵巢癌中的表达情况及临床意义。方法:收集Oncomine、GEPIA数据库中关于MECOM的数据信息,并对数据库中的资料进行二次分析;运用cBioportal、Coexpedia、STRING数据库分析MECOM在卵巢癌中的突变、共表达及互作蛋白情况;利用Kaplan-Meier Plotter分析卵巢癌患者MECOM mRNA的表达水平与患者预后的关系。结果:Oncomine数据库中对不同类型肿瘤有关MECOM基因的研究共353项,差异有统计学意义的有99项(高表达33项,低表达66项),其中对卵巢癌研究有10项,均显示MECOM高表达;这一结果在GEPIA数据库中也得到证实。突变分析结果显示卵巢癌患者中有30%的患者MECOM基因发生突变,突变类型包括错义突变、融合突变和截断突变。共表达分析发现了275个与其共表达的基因;蛋白互作分析发现MECOM蛋白可能与NR2E1、MTA1、HDAC1、TP53、PPARG、EZH2、JUN、MBD3、HDAC2和CTBP1蛋白有相互作用。MECOM mRNA高表达组卵巢癌患者总生存期低于低表达组(P=0.013)。结论:MECOM基因在卵巢癌中高表达,且其翻译的蛋白通过和多种蛋白相互作用发挥生物学效应,其mRNA表达水平与卵巢癌患者生存预后相关,有望为卵巢癌分子靶向治疗提供新思路。

Objective:To explore the expression and its clinical significance of MECOM in ovarian cancer.Methods:The data about MECOM was collected from Oncomine and GEPIA databases;cBioportal,Coexpedia and STRING database were used to analyze the mutation,co-expression and interacting proteins of MECOM in ovarian cancer.Kaplan-Meier Plotter was used to analyze the relationship between the expression level of MECOM mRNA and the prognosis of patients with ovarian cancer.Results:With different types of cancer in Oncomine database,there were 353 studies related to MECOM,and 99 of them showed statistically significance(33 high-regulated and 99 low-regulated),including 10 studies on ovarian cancer,both show high MECOM expression.This result was also confirmed in the GEPIA database.Mutation analysis showed that 30%of ovarian cancer patients had mutations in MECOM gene,including missense mutation,fusion mutation and truncation mutation.Co-expression analysis showed that 275 genes were co-expressed.MECOM may interact with NR2E1,MTA1,HDAC1,TP53,PPARG,EZH2,JUN,MBD3,HDAC2 and CTBP1.The total survival rate of ovarian cancer patients in the group with high MECOM mRNA expression was lower than that with low MECOM mRNA expression(P=0.013).Conclusions:MECOM gene is overexpression in ovarian cancer,and its translated proteins interact with various proteins to exert biological effects.The expression level of MECOM mRNA is related to the survival prognosis of ovarian cancer patients,which is expected to provide a new idea for molecular targeted therapy of ovarian cancer.