子痫前期患者与正常孕妇血清脂质组学成分对比分析

Comparative Analysis of Serum Lipid Components between Pre-Eclampsia Patients and Normal Pregnant Women

目的:对子痫前期(pre-eclampsia,PE)患者和正常孕妇血清中脂质成分进行对比分析。方法:纳入西安医学院第一附属医院产科2019年1月1日—7月1日收治的PE患者16例为PE组,选取同期自然分娩正常孕妇16例为正常对照组(NC组),收集2组患者血清。采用超高效液相色谱串联质谱(ultra-performance liquid chromatography tandem mass spectrometry,UPLC-MS/MS)法对各组患者血清中的甘油磷脂、鞘脂、甘油酯、甾醇类及脂肪酸五大类脂质成分进行分析。结果:2组孕妇年龄和采血时体质量指数(BMI)差异无统计学意义(P>0.05)。PE组患者妊娠时间低于NC组(P<0.05)。对2组患者血清中5大类脂质共计25种脂质成分进行分析,相比于NC组,PE组的甘油磷脂、鞘脂、甘油酯和甾醇类表达差异无统计学意义(P>0.05)。PE组患者血清中游离脂肪酸(free fatty acid,FFA)含量高于NC组,差异有统计学意义(P<0.05)。进一步对13种不同类型FFA进行分析,发现PE组孕妇血清中FFA22:4、FFA20:5、FFA20:4、FFA20:3、FFA18:2、FFA18:1、FFA16:1、FFA16:0和FFA14:0与NC组相比显著升高,差异有统计学意义(P<0.05)。结论:PE患者血清中出现多种不同类型的FFA的差异化表达,不同类型FFA可能成为疾病诊断的预警分子,在疾病预测及机制探索中具有一定作用。

Objective:To compare and analyze the serum lipid components of pre-eclampsia(PE)patients and normal pregnant women.Methods:A total of 16 patients with pre-eclampsia admitted to the obstetrics department of the First Affiliated Hospital of Xi′an Medical University from January 1,2019 to July 1,2019 were included as PE group.Sixteen cases of normal pregnant women were selected as the normal control group(NC group),and serum of the two groups were collected.Five kind of lipid as glycerolipids,spingolipids,glycerides,sterols and fatty acids in serum of each group were analyzed by ultra-performance liquid chromatography tandem mass spectrometry(UPLC-MS/MS).Results:There were no significant differences in the age and BMI of between the two groups(P>0.05).The gestational age of PE group was significantly decreased than that of NC group(P<0.05).A total of 25 lipid components were analyzed in serum of the two groups,and the results showed that compared with the NC group,the expression of glycerolipids,sphingolipids,glycerides and sterols in PE group had no statistical significance(P>0.05).The serum fatty acid content in PE group was significantly higher than that in NC group,and the difference was statistically significant(P<0.05).Further analysis of 13 different types of fatty acids showed that the serum levels of FFA22:4,FFA20:5,FFA20:4,FFA20:3,FFA18:2,FFA18:1,FFA16:1,FFA16:0 and FFA14:0 in pregnant women in PE group were significantly higher than those in NC group.The difference was statistically significant(P<0.05).Conclusions:Different types of fatty acids are differentially expressed in serum of PE patients.The above different types of FFA may be the early-warning molecules for disease diagnosis and play a certain role in disease prediction and mechanism exploration.