应用实时荧光定量PCR检测湖州市鼠形动物汉坦病毒感染状况

An investigation of Hantavirus infection in rodents by real-time quantitative PCR in Huzhou City

目的调查湖州市城郊鼠形动物分布,分析鼠形动物汉坦病毒感染状况。方法 2020年9月—11月在湖州郊区捕获鼠形动物,采集肺组织提取核酸,应用实时荧光定量PCR方法检测汉坦病毒病原体。结果共捕获鼠形动物105只,共检出阳性动物11只,感染率为10.48%。不同生境鼠形动物的汉坦病毒感染率差异无统计学意义(P=0.052),重点行业鼠形动物汉坦病毒阳性率为30.77%(4/13),城镇居民区为11.11%(1/9),农田耕地为8.82%(6/68),农村居民区未检出汉坦病毒。105只鼠形动物中黑线姬鼠39只、黄胸鼠37只、褐家鼠6只、中华姬鼠3只和鼩鼱20只。褐家鼠感染率为16.67%(1/6),黄胸鼠感染率为13.51%(5/37),黑线姬鼠感染率为12.82%(5/39),鼩鼱和中华姬鼠未检出汉坦病毒。结论湖州地区鼠形动物汉坦病毒感染率较高,应加强动物宿主和人群的病原学调查等针对性防制工作。

Objective To investigate the distribution of rodents in the suburbs of Huzhou and to analyze the hantavirus infection status of rodents.Methods From September to November of 2020, the rodents were collected in suburban areas of Huzhou.Nucleic acid was extracted from lung tissue and the pathogen of Hantavirus was detected by real-time quantitative PCR.Results A total of 105 rodents were captured, and the infection rate of Hantaan virus in 11 detected rodents was 10. 48%.There was no statistical significance on the difference in the infection rate of Hantavirus among rodents in the same habitats(P= 0. 052). The positive rate of Hantavirus in rodents in key industries was 30. 77%(4/13), that in urban residential areas was 11. 11%(1/9), and that in farmland was 8. 82%(6/68). No Hantavirus was detected in rural residential areas. 105 rodents included 39 apodemus agrarius, 37 rattus flavipectus, 6 sewer rat, 3 apodemus draco and 20 shrews. The infection rate was 16. 67%(1/6) in sewer rat, 13. 51%(5/37) in rattus flavipectus, 12. 82%(5/39) in apodemus agrarius, and no Hantavirus was detected in shrew and apodemus draco.Conclusion The infection rate of Hantavirus in rodents is high in Huzhou area. We should further strengthen the etiological investigation of animal hosts and people.